I agree with Paul and George that it is vital that PDB files from TLS
refinement contain ANISOUs which reflect the results of that refinement,
and would like to reinforce Paul's point below.
There are many possible parameter-frugal approximations to full
ADP-refinement: TLS is just one of
Dear [EMAIL PROTECTED],
I have to correct my earlier statement in an previous email to the
bulletin board (see below): One of the detection channels of the AB
StepOne is in the right detection range and the signal is strong!
Our lab just purchased the new AB StepOne RT-PCR machine for
We do have to mess with the PDB format every time something new is
invented. Usually it's just REMARK 3 that has to be changed, but you
always need to put some kind of remark in the header that you used a
certain type of refinement.
When different types of anisotropic refinement are combined
Call for access to Synchrotron Beamline Facilities 2008
We announce a call for synchrotron beam time applications in biological
X-ray crystallography (PX) and small-angle scattering (SAXS).
Up to 12 weeks of beam time will be available at the DORIS storage ring
(DESY) during the period
POSTDOCTORAL POSITION IN PROTEIN CRYSTALLOGRAPHY OF MITOCHONDRIAL OUTER
MEMRBANE COMPLEXES
The objective of this position is to determine the atomic structure of
complexes from the outer mitochondrial membrane. Our goal is to understand the
crosstalk of mitochondria with the cytosol via
On Sun, Mar 30, 2008 at 07:08:14PM +0100, Partha Chakrabarti wrote:
Is it by any chance that the FOMs were highly overestimated and that
creates a problem with Maximum likelihood? That sort of reminds me of
what I had heard for SHARP-solomon in a couple of instances..
The FOMs are never used
In the end, we're solving all these structures because we believe (or
at least hope) that they'll be useful for understanding
biology. That means that biologists should be able to understand
what we deposit.
When I've tried to teach undergraduates what to make of structural
models, I find I
Hi,
On Sat, Mar 29, 2008 at 07:57:44PM -, Martyn Winn wrote:
Anyway, these are all different representations of the same thing,
and should work equally well so long as you know which you are
using. The scariest thing from the last thread was that our attempt
to document it with a REMARK 3
I am not mistaken what went wrong in my case is that
in resolve.mtz there are the columns FP and SIGFP which are not the
experimental F anymore
but they are modified ones (solvent flattened, ncs etc...).
Clearly refmac5 uses them as Fo for refinement, refining Fc against Fc (an
extreme case of
[EMAIL PROTECTED]
A[EMAIL PROTECTED]
[EMAIL PROTECTED]
From: Ian Tickle [EMAIL PROTECTED]
To: Winn, MD (Martyn) [EMAIL PROTECTED], CCP4BB@JISCMAIL.AC.UK
Return-Path: [EMAIL PROTECTED]
X-OriginalArrivalTime: 31 Mar 2008 16:34:19.0370 (UTC)
FILETIME=[1165ACA0:01C8934D]
On Monday 31 March 2008 08:58, [EMAIL PROTECTED] wrote:
In the end, we're solving all these structures because we believe (or
at least hope) that they'll be useful for understanding
biology. That means that biologists should be able to understand
what we deposit.
Agreed, but...
When
True enough. My comment was in the context of whether it is better to
deposit ANISOU lines as a representation of the anisotropy.
The default for BRES in TLSANL is indeed false. This was originally done
so as not to break the pre-existing RESTRAIN usage. This is taken care
of in the GUI, and for
[EMAIL PROTECTED] wrote:
In the end, we're solving all these structures because we believe (or at
least hope) that they'll be useful for understanding biology. That
means that biologists should be able to understand what we deposit.
The problem is that we seem to want a format which is
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