Re: [ccp4bb] B factor calculation

2008-06-05 Thread Mark J. van Raaij
in ccp4, baverage does the job. Mark Mark J. van Raaij Dpto de BioquĂ­mica, Facultad de Farmacia Universidad de Santiago 15782 Santiago de Compostela Spain http://web.usc.es/~vanraaij/ On 4 Jun 2008, at 21:40, xu zhen wrote: Hi, everyone, I am preparing the table of data collection and

Re: [ccp4bb] crystallisation and mosaicity

2008-06-05 Thread Juergen Bosch
Hi Charlie, yes you are right, but I assumed if people see a cloud of condensed fog over their LN2 bath they should remove that by a) filling up the bowl completely e.g. some LN2 drips out of the bowl b) blow the fog away before you dip c) ask someone for advice In general I have observed

[ccp4bb] Fwd: [ccp4bb] crystallisation and mosaicity

2008-06-05 Thread Patrick Loll
Hi Charlie, yes you are right, but I assumed if people see a cloud of condensed fog over their LN2 bath they should remove that by a) filling up the bowl completely e.g. some LN2 drips out of the bowl b) blow the fog away before you dip True; this has been demonstrated quite rigorously:

Re: [ccp4bb] 3D model building without CRT monitor

2008-06-05 Thread Vangelis Christodoulou
Philips is offering an interesting solution: http://www.business-sites.philips.com/3dsolutions/products/3dscreens/index.html Vangelis On Jun 2, 2008, at 2:10 PM, Krojer,Tobias wrote: Dear all, recently some of our CRT monitors broke down and we realized that these monitors are no longer

Re: [ccp4bb] 3D model building without CRT monitor

2008-06-05 Thread Dima Klenchin
Vangelis Christodoulou wrote: Philips is offering an interesting solution: http://www.business-sites.philips.com/3dsolutions/products/3dscreens/index.htmlhttp://www.business-sites.philips.com/3dsolutions/products/3dscreens/index.html Interesting! Their PDF says it works with OpenGL:

Re: [ccp4bb] Fwd: [ccp4bb] crystallisation and mosaicity

2008-06-05 Thread Petr Leiman
yes you are right, but I assumed if people see a cloud of condensed fog over their LN2 bath they should remove that by a) filling up the bowl completely e.g. some LN2 drips out of the bowl b) blow the fog away before you dip I think the original poster meant the relatively low heat conduction

Re: [ccp4bb] 3D model building without CRT monitor

2008-06-05 Thread Juergen Bosch
Dima Klenchin wrote: Vangelis Christodoulou wrote: Philips is offering an interesting solution: http://www.business-sites.philips.com/3dsolutions/products/3dscreens/index.htmlhttp://www.business-sites.philips.com/3dsolutions/products/3dscreens/index.html Interesting! Their PDF says it

Re: [ccp4bb] crystallisation and mosaicity

2008-06-05 Thread Lokesh Gakhar
Charlie, The insulating gas cushion is due to the Leidenfrost effect ( http://en.wikipedia.org/wiki/Leidenfrost_effect). We discussed this somewhat in: J. Appl. Cryst. (2005). 38, 945-950 -Lokesh On Wed, Jun 4, 2008 at 8:11 PM, Charlie Bond [EMAIL PROTECTED] wrote: I seem to recall an

Re: [ccp4bb] Fwd: [ccp4bb] crystallisation and mosaicity

2008-06-05 Thread Edward Berry
I think the important thing here is that liquid nitrogen in the lab tends to be exactly at its boiling point, since the temperature is maintained by continuously boiling off some of the N2. This means the only mechanism for heat absorption is through vaporization, depending on the latent heat of

Re: [ccp4bb] 3D model building without CRT monitor

2008-06-05 Thread Vangelis Christodoulou
There is a 20-inch (20-3D2W04) display that costs around $6000. For $13000 you get a 42-inch, so I guess the 52-inch that was introduced today will cost a lot. Vangelis On Jun 5, 2008, at 7:30 PM, Juergen Bosch wrote: Interesting! Their PDF says it works with OpenGL:

[ccp4bb] analytical ultracentrifugation

2008-06-05 Thread Michael Colaneri
Dear colleagues, does anyone know of a faciltiy for analytical ultracentrifugation? Thanks. Mike Colaneri

[ccp4bb] AstraZeneca US permanent PX position

2008-06-05 Thread Read, Jon
I would like to draw your attention to a protein crystallography position advertised on the AstraZeneca jobs web pages. This is a permanent position in a young structural biology team being established at our site in Boston, Massachussets. The position would suit an all-round, hands-on

Re: [ccp4bb] analytical ultracentrifugation

2008-06-05 Thread verdino
Hi Mike, try the National Analytical Ultracentrifugation Facility in Connecticut. http://www.biotech.uconn.edu/auf/ Cheers, Petra Dear colleagues, does anyone know of a faciltiy for analytical ultracentrifugation? Thanks. Mike Colaneri -- Petra Verdino, PhD Research Associate The

Re: [ccp4bb] Fwd: [ccp4bb] crystallisation and mosaicity

2008-06-05 Thread Tommi Kajander
according to literature,see below and references http://www.px.nsls.bnl.gov/courses/papers/ZD_EG_papers.html, it is not clear that liq. propane plunged item would cool faster. (whilst i havent tested this)... Would anyone have actual experimental data with protein crystals on the

Re: [ccp4bb] 3D model building without CRT monitor

2008-06-05 Thread PhilEvans
We now have a Planar system, which works very nicely with coot, O , ccp4mg etc. Rather expensive though Phil Evans On 2 Jun 2008, at 17:06, Mike Latchem wrote: These new TFT monitors do seem like a really good idea, but, it is well worth doing in depth research as most of the accessible

Re: [ccp4bb] analytical ultracentrifugation

2008-06-05 Thread weikai
Hi: Just a related question. To measure MW of membrane proteins, the density of detergents needs to be matched by D2O/H2O and should be within 1.0 to 1.1. Anyone knows some literature that gives a list of detergents having suitable density? Unfornatatedly, the common ones like Foscholine

Re: [ccp4bb] 3D model building without CRT monitor

2008-06-05 Thread James M. Vergis
Has anyone tried a 3D DLP-TV? They have the input for a stereographics emitter. Probably too big for a desktop but at 50-72 they would work in a conference room. They range in price from $1900-3500. Here is the link for the SAMSUNG website http://product.samsung.com/dlp3d/index.html.