Re: [ccp4bb] CCP4MG can't start

Hi Bill, I commented out the export DISPLAY lines, but it didn't help. What did fix it was reinstalling x11 from the OSX 10.5 CD. Any ideas? I've attached the launch script as a text file. Thanks! Kendall On 7/25/08 12:27 PM, William G. Scott [EMAIL PROTECTED] wrote: Hi Kendall: I haven't

[ccp4bb] rcss, how to

Hi every one, Please, I need help to calculate the rcss. Thnaks in advanced, Ariel -- Ariel Talavera, Lic. Dept. of Computational and Structural Biology Center of Molecular Immunology P.O.Box 16040, Havana 11600 Cuba tel: (53-7) 271 7933, ext. 219 fax: (53-7) 272 0644 email: [EMAIL PROTECTED]

[ccp4bb] carrection, rscc

Hi again, I am trying to calculate the rscc of the model to the electron density map. Any help will be appreciated. Thanks, Ariel -- Ariel Talavera, Lic. Dept. of Computational and Structural Biology Center of Molecular Immunology P.O.Box 16040, Havana 11600 Cuba tel: (53-7) 271 7933, ext.

[ccp4bb] sfcheck output file and rscc

Hi there, Following the instructions I got from the mail list (thanks to them) I ran sfchek to get the rscc. When I did it I could nor find the exact term of rscc but there is a Correlation Factor into the Model vs. Structure Factor chart. Is that Correlation Factor the rscc I am looking

[ccp4bb] Beamline Stability Issues

Hello Michele! Thanks for your answer!... Answering your questions we have a collimating mirror upstream the mono and the water temperature used to keep this guy cooled is within +/-0.3°C. Regarding fluctuation in the water flow, I don’t know, maybe not ‘cause we have a dedicated thermal bath

Re: [ccp4bb] Refinement problem

Hi Sampath, On Sat, Jul 26, 2008 at 02:05:17AM +0900, Sampath Natarajan wrote: Also I could find many cuts in the density. This looks to me like a problem with your low-resolution data? Since you collected 1.6A data my guess is that you probably had a fair amount of overloads. Did you do a

[ccp4bb] off-topic: protein crystallography in organic chemistry lab

Dear all, Sorry for an extremely off-topic question, but I thought this would be the best place to find an answer. We are setting up our protein crystallography lab and have been asked to share laboratory space with an organic chemistry group which is already well established in the room.

Re: [ccp4bb] question about getting rid of model bias in refinement

If resolution is around 2.0 or better, ARP/wARP is particularly powerful for such cases when model bias needs to be reduced. For more info: http://www.ncbi.nlm.nih.gov/pubmed/18094467?ordinalpos=1itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum

Re: [ccp4bb] off-topic: protein crystallography in organic chemistry lab

Hi, I assume that there is no other way and you have to share the space, so I will save you from the standard tirade about functional space segregation :-) If the air in the lab is so loaded with solvents that your plates are melting - then *you* (or anyone else for that matter) certainly

[ccp4bb] estimate protein in Incl bod

We have our recombinant protein expressed in inclusion bodies we just wash the IBs and perform solubilization and refolding. Our solubilisation protocol mentions making up the volume to contain 7-8 mg / ml protein. 1. how can we determine the total protein content in the inclusion bodies

Re: [ccp4bb] estimate protein in Incl bod

You can solibilize IB with your solublization buffer, measure the solubilized protein concentration by a protein assay like Bradford. Then adjust the concentration. Most weight of IBs is not from proteins. However, your protein should be the predominant protein in the IB if the expression is done