Dear all,
I was wondering if anyone had some practical advice in regards to increasing
the size of a crystal. Currently my enzyme forms these rather nice cubic and
very sharp 0.1mm (in 25% peg 5000, 0.1M ammonium sulphate, 0.1M Tris ph
7.0) crystals following optimisation of the pH and
Anything that slows growth potentially could help.
In one case, I found by re-dissolving drops with showers of small
crystals I could get one or two huge ones. I just added 2 microliters
of water to the handing drop and allowed it to re-equilibrate.
Changing temperature or any physical
We've had lots of success with the additive screens (3 boxes of 24
additives) sold by Hampton Research
Vincenzo Carbone wrote:
Dear all,
I was wondering if anyone had some practical advice in regards to
increasing the size of a crystal. Currently my enzyme forms these
rather nice cubic
I feel compelled to add: rather nice cubic 0.1mm presumably means
they're 90x90x90um? At any state-of-the-art synchrotron beamline with a
half-way decently-sized beam, that's an absolute stonker, we rarely
optimize more if they're that large.
phx.
Vincenzo Carbone wrote:
Dear all,
I
Oh, another thing you can try is simply increasing the drop volume,
especially large sitting drops. This will slow down equilibration,
and there will be proportionately more material
On Sep 9, 2008, at 11:23 PM, Vincenzo Carbone wrote:
Dear all,
I was wondering if anyone had some
-Original Message-
From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED]
On
Behalf Of Eleanor Dodson
Sent: 09 September 2008 17:11
To: William G. Scott
Cc: CCP4BB@jiscmail.ac.uk
Subject: Re: [ccp4bb] truncate and anisotropy
I dont think you need to worry much about the strong
Hi Vince,
There is a very nice paper by Monika Budayova-Spano in Acta Cryst 63D
2007 339-347. A methodology and an instrument for the temperature-
controlled optimization of crystal growth.
Best,
Wally
On Sep 10, 2008, at 2:23 AM, Vincenzo Carbone wrote:
Dear all,
I was wondering if
My usual first approach to increasing crystal size is to decrease
precipitant and increase protein concentration, keeping the mixture in
the proper nucleation zone, but providing more protein for crystal
growth. Minimizing dust or particulates by filtration and centrifugation
of reagents and
Hi!
I would suggest to try another temperature or seeding. It worked for me before.
Good luck!
Nurit.
Anyone who has never made a mistake has never tried anything new. Albert
Einstein
Nurit Mirkin Bril Date: Wed, 10 Sep 2008 08:02:53 -0400 From: [EMAIL
PROTECTED] Subject: Re: [ccp4bb]
Dear all,
I am looking for a program that can rotate atoms with anisotropic B factors
- not only change coordinates of the atom, but to modify the Anisou
coefficients too.
If you are confident that there is none let me know too ;-)
Pavel
Pavel
There is a program from the computer stone-age called XP (nothing to do
with Microsoft who though of the name many years later) that does exactly
what you need (with the SGEN instruction). It is part of the Bruker
SHELXTL software, maybe you can find a nearby small-molecule
Laboratory Technician Position at the CIC bioGUNE
(Bilbao-Spain)
Applications are invited for a Laboratory Technician
in the group of Dr. Nicola G. A. Abrescia at the
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To the CCP4bb members who are/have been users of Art Robbins' Phoenix robot:
Would anyone be interested in joining a Phoenix robot user group/bulletin
board? Someplace where users would be able to discuss their experiences, as
well as to learn from the experiences of others.
I will be
Hi all,
thanks a lot for all the replies! Using the very current version of XDS
fixed all the problems, really nice data, structure solved, yet another one
for SSGCID.
re input file: attached the XDS.INP file that worked for the Saturn 944+
detector.
Thanks again!
Jan
2008/9/4 Jan Abendroth
Well, I was pointing to the Sivia David (1994) paper because I thought
it might be helpful in the discussion about how to convert intensities
to amplitudes. The paper is probably not so well known in the PX
community, so I decided that I would advertise it on this BB. However,
since I am not one
Hi all (sorry, don't know where else to post, suggestions welcome)
We have for sale a 3 year-old Rigaku LN40 liquid nitrogen generator,
which we no longer need thanks to new house lN2 supply. For a picture,
see here:
http://www.rigaku.com/cryo/nitrogen.html
It was working
Hi,
Whatever happened to the Yahoo group pxrbtx (PX robotics), that was
started by Ingo Koendorfer in 2006? There haven't been any postings
since Feb. 2007. Maybe time to revive it?
Derek
On Sep 10, 2008, at 15:19, Critton, David wrote:
To the CCP4bb members who are/have been users of
We would like to invite applications for postdoctoral position in Prof.
Senyon Choe's Structural Biology Laboratory at The Salk Institute
(http://sbl.salk.edu/~choe) to study structures of membrane proteins.
Strong background in membrane protein biochemistry is required.
Those interested should
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