Hi Cedric,
I used these, but just for testing crystals. I was afraid that the
crystal might move in the loop. For testing it worked pretty good.
http://www.jenabioscience.com/cms/en/1/catalog/733_microrttrade_room_temperature_mounting_system.html
There might be more suppliers. Please send a
http://www.mitegen.com/
Cheers
Carsten
-Original Message-
From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk]on Behalf Of cedric
bauvois
Sent: Friday, January 16, 2009 9:27 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] cryoloops for X-ray data collection from protein
cedric bauvois wrote:
Dear CCP4ers,
in their paper entitled Using cryoloops for X-ray data collection from
protein crystals at room temperature: A simple applicable method (
*Journal of Crystal Growth*
http://www.sciencedirect.com/science/journal/00220248
Volume 281, Issues 2-4
Hi Cedric,
I haven't read this paper, but there's already a system available for roomtemp
data collection that works quite well. Check out
http://www.mitegen.com/products/micrort/micrort.shtml
Instead of a capillary they use thin polyester tubing that you slide over
(special) bases so that
You lose about 1 microlitre of water per well per day with polystyrene
Linbro plates - it goes through the plastic
Polypropylene and COC are better (or maybe worse if the evaporation
encourages crystallization)
--
For information and discussion about protein crystallization and
automation,
If you just want to check crystals at room temperature, we have
developed a Direct Ex Plate after Acta Cryst. D58(10), 1527-1530
(2002).
Please check,
http://www.labo.co.jp/contents/direct_ex.html
(Sorry for Japanese)
Nobuhisa Watanabe
===
Synchrotron Radiation Research Center
Department of
Dear Cedric,
We use a much easier test for mounting crystals at room temperature:
just coat the crystal with paratone oil and mount your crystal in a standard
cryoloop. The oil will slow down evaporation enough - no special tools
required. You don't even need to remove all the liquid as you
Hello Jacob,
The problem for native gel is that it is much more sensitive to a
single charge difference than size differences. Also, the gel pattern
may change greatly if you use a different buffer system. I had a case
2 years ago that my protein ran at 5 positions on a Laemmli(pH 8.8)
