Re: [ccp4bb] Posttranslational modifications of recombinant proteins expressed in E. coli?

2009-01-20 Thread Anthony Addlagatta
Alfredo, The other alternative explanation to your two species protein sample is in the presence or absence of initiator methionine. About 70-80 of all prokaryotic proteins under post-translational removal of initiator formylmethioine (178 Da) in two step process. First the formyl (29 Da)

[ccp4bb] CALL for PROPOSAL on ESRF BIO-SAXS BEAMLINE ID14-3

2009-01-20 Thread nurizzo
PROPOSALS FOR BEAMTIME ON THE ESRF Bio-SAXS BEAMLINE ID14-3 User operation on the new Bio-SAXS beamline at ID14-3 at the ESRF ( http://www.esrf.fr/UsersAndScience/Experiments/MX/About_our_beamlines/copy_of_ID14-3 ) began in November 2008 with mounting of samples currently performed manually.

Re: [ccp4bb] Raxis II - free to good home!

2009-01-20 Thread Pamela Focia
We have found a home for our system. Thanks to all who replied. At 2:06 AM + 1/20/09, Pamela Focia wrote: We have an Raxis II system we took down a few months ago, plus an almost full, duplicate set of its major parts that we salvaged from a neighboring University last year. If you can

[ccp4bb] Replacement for arp_waters?

2009-01-20 Thread David J. Schuller
I note that in CCP4 6.1.0, arp_waters has been deprecated. Is there a program in the suite which fills the role formerly filled by arp_waters, of automatically adding waters? Cheers, - === You can't possibly be a scientist if

Re: [ccp4bb] Replacement for arp_waters?

2009-01-20 Thread Pavel Afonine
Hi David, for example: phenix.refine does it automatically as part of usual refinement; for illustration, see the picture here: http://phenix-online.org/documentation/refinement.htm Cheers, Pavel. On 1/20/2009 10:13 AM, David J. Schuller wrote: I note that in CCP4 6.1.0, arp_waters has

[ccp4bb] ccp4 download problems

2009-01-20 Thread Alessandro Nascimento
Is anybody else having problems to download ccp4 from automated page? I can only get a 70Kb file, which I don't believe contains all the package! Thanks in advance -- [ ]s --alessandro

Re: [ccp4bb] Replacement for arp_waters?

2009-01-20 Thread Roger Rowlett
I will second noting the loss of arp_waters in CCP4 6.1. I know there is a "find waters" option in Coot 0.5.2 under "Calculate...Other modeling tools", and perhaps this is adequate, but it doesn't seem to have as many options for fine-tuning as arp_waters did. Coot allows for finding waters

Re: [ccp4bb] Replacement for arp_waters?

2009-01-20 Thread Bernhard Rupp
arp_waters is very useful for a variety of purposes. I would like to see it supported in future releases. Best, BR From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of Roger Rowlett Sent: Tuesday, January 20, 2009 11:40 AM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb]

[ccp4bb] ccp4 downloads pages

2009-01-20 Thread Charles Ballard
Dear All Packages downloaded since 10 am GMT will be missing the core suite, and other elements. This problem has now been fixed, and everything should be working properly. Sorry for any inconvenience Charles Ballard CCP4

Re: [ccp4bb] Replacement for arp_waters?

2009-01-20 Thread Paul Emsley
Roger Rowlett wrote: I will second noting the loss of arp_waters in CCP4 6.1. I know there is a find waters option in Coot 0.5.2 under Calculate...Other modeling tools, and perhaps this is adequate, but it doesn't seem to have as many options for fine-tuning as arp_waters did. Coot allows for

Re: [ccp4bb] Create a dual boot windows-linux machine

2009-01-20 Thread Ed Pozharski
And Linux partitions can be accessed from windows: http://www.howtoforge.com/access-linux-partitions-from-windows On Mon, 2009-01-19 at 12:49 +, Roger Dodd wrote: Dear all, Just to add, linux now has perfectly good read-write stable drivers for NTFS (the native WinXP filesystem). This

Re: [ccp4bb] Replacement for arp_waters?

2009-01-20 Thread Winn, MD (Martyn)
Part of the reasoning was that arp_waters corresponded to a very old version of arp/warp It was felt that up-to-date arp/warp and/or findwaters covered the functionality. Of course, every implementation does things in a slightly different way, and each has their fans. Which means it is hard to

[ccp4bb] 3D 120hz LCD setups available

2009-01-20 Thread Francis E Reyes
Since this was of discussion on this board a week or so ago, I figure it has relevancy. http://store.nvidia.com/DRHM/servlet/ControllerServlet?Action=DisplayProductDetailsPageSiteID=nvidiaLocale=en_USEnv=BASEproductID=111286400utm_source=newsletterutm_medium=emailutm_campaign=store_Jan09#

Re: [ccp4bb] Replacement for arp_waters?

2009-01-20 Thread Soisson, Stephen M
Might I add yet another call for the reinstatement of arp_waters - a very useful program indeed. Steve From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of Bernhard Rupp Sent: Tuesday, January 20, 2009 2:50 PM To: CCP4BB@JISCMAIL.AC.UK Subject:

Re: [ccp4bb] Replacement for arp_waters?

2009-01-20 Thread Donnie Berkholz
On 17:05 Tue 20 Jan , Paul Emsley wrote: Roger Rowlett wrote: I will second noting the loss of arp_waters in CCP4 6.1. I know there is a find waters option in Coot 0.5.2 under Calculate...Other modeling tools, and perhaps this is adequate, but it doesn't seem to have as many options

Re: [ccp4bb] Replacement for arp_waters?

2009-01-20 Thread Victor Alves
* * * As a newbie, am I stating the obvious or missing something:   What about installing ARPwARP 7.0.1 in CCP4 (really easy!)?   You?ll then have in CCP4i /Model Building, the ARP/wARP Solvent Module     Victor Alves Quoting David J. Schuller dj...@cornell.edu:

Re: [ccp4bb] Replacement for arp_waters?

2009-01-20 Thread Dima Klenchin
Donnie Berkholz wrote: One of the major reasons our lab started using Coot instead of O is that you didn't have to memorize or look up a million obscure functions to do everything. It was in the Coot GUI. When we're training new people, this helps immensely for the 99% of them who have never

Re: [ccp4bb] 3D 120hz LCD setups available

2009-01-20 Thread Zhijie Li
I remember that the Geforce series are capable of doing stereo only under the fullscreen OpenGL mode - which is only used in 3D games. This capability is provided by the Nvidia video driver. Under this mode, the card takes the 3D model that the game software sends to it, and generates a stereo

[ccp4bb] POSTDOCTORAL POSITION IN STRUCTURAL BIOLOGY AT UNIVERSITY OF TOULOUSE

2009-01-20 Thread Valerie Guillet
*POSTDOCTORAL POSITION IN STRUCTURAL BIOLOGY * INSTITUT DE PHARMACOLOGIE ET DE BIOLOGIE STRUCTURALE CNRS, UNIVERSITE DE TOULOUSE FRANCE A postdoctoral position is immediately available in the group of Lionel Mourey at the Institut de Pharmacologie et de Biologie Structurale (IPBS) to