Re: [ccp4bb] ARP/wARP and computer platforms

2009-02-21 Thread John Badger
Interesting that these questionaire responses are very different from the numbers obained from looking at downloads counts for other cross-platform crystallography software. I wonder if they are more reflective of the currently available ARP-wARP platforms and perhaps the role of administrator- pr

[ccp4bb] Fwd: more about low order reflections

2009-02-21 Thread Richard Gillilan
Since Sacha is having trouble posting directly the list I will forward his latest message since It addresses my second set of questions: Begin forwarded message: From: Alexandre OURJOUMTSEV Thank you, Richard, for your questions ! Unfortunately, I failed to pass my mail to the CCP4bb - I

Re: [ccp4bb] Could I improve an inconsecutive experimental map?

2009-02-21 Thread Charlie Bond
Hi Alphar, A while ago I had success with a poor MIR map by building in a few fragments I could identify and then combining phases from this model with the MIR phases using SIGMAA (CCP4 program) and then calculating new maps. A couple of cycles of this resulted in phases in which I had con

Re: [ccp4bb] Could I improve an inconsecutive experimental map?

2009-02-21 Thread Fengyun Ni
Thanks, Ezra! I have a native dataset. I'll try what you said, Hope it works in my case. Thanks! Alphar 2009/2/21 Ezra Peisach > Personally, I would try to build into what you see and then do phase > recombination. I had a SeMet crystal in which I could only see part of a > sheet and helix

Re: [ccp4bb] Could I improve an inconsecutive experimental map?

2009-02-21 Thread Fengyun Ni
Thanks, Poul! How could I change the scale of the data intensities by a factor 2-3? Do I need to change it in scaleit or to change it in mlphare? Is that always necessary to make the occupancy of the major site to become ~1? I'll try this. I use phenix to detect the twin effect, the results turned

Re: [ccp4bb] Could I improve an inconsecutive experimental map?

2009-02-21 Thread Poul Nissen
Hi alphar~ Check the sites you get from anomalous difference Fouriers and stick to those as your correct HA sites. At the same time you may consider not to refine the HA substructure on the anomalous differences but merely using the isomorphous differences. Maybe you need to change the sca