Hi Andrzej,
Thanks for the tip. It just seemed to me that since the package
installed as a typical Mac OS X clickable program, then it was meant
to run that way. Thanks also for info about adxv. I wasn't aware that
there was a Mac executable for this program.
Kay, the image I was trying t
Hi,
Once you have a 2cbf in /usr/local/bin and start XDS Viewer from a
terminal (/Applications/XDS-Viewer.app/Contents/MacOS/XDS-Viewer) it
seems to work fine.
However for the diffraction image viewing I would suggest adxv. You
can find it at: http://www.scripps.edu/~arvai/adxv.html.
And
Hi all,
I keep on getting the following error message when I try to start a
refmac job through ccp4i with ccp4 version 6.1.1. This initially only
happened in a linux installation, now all of a sudden appeared in a
osx installation as well. The interface hangs when starting the job.
It also
I had the same problem. In my case I think it was related to the part
of ccp4 configuration that redefines python path. For some reasons (it
breaks wxPython, at least in Hardy), I had it commented out and then
after starting ccp4i I got these dbccp4i warnings. When I turned python
part of ccp4 c
David Garboczi wrote:
Have large proteins (300+ kD) been successfully produced in E. coli?
Which ones?
have a look at Suo et al., 2000, PNAS, 14188-14193; (350 kDa);
or Pan et al., 1999, NAR, 1094-1103; ('only' 190 kDa).
klaus
Dave
--
Hi Raji-
A number of GPCRs have been overexpressed in E. coli. Examples are Reinhard
Grishammer's work with neurotensin receptor and Christopher Tate's work with
B2-adrenergic and adenosine 2a receptors. The problem was not necessarily
expression but proper outer membrane targeting of *active* rece
Paul Emsley wrote:
And for those who like the command line to convert .res to .pdb (and
have Coot):
[see attached]
Oh curses - sorry. Not everyone develops coot.
s?\./coot-real?coot?
Paul.
Hi there,
The human protein FLAP from the MAPEG family was expressed in E. coli
and also the K-channel, KirBac1.3 (although this is a chimera of mouse
and B. xenovorans).
The pubmed id codes for related papers are: 17703190 and 17600184
and the pdb codes for the structures are: 2QKS and 2Q
Hi,
I very recently downloaded and installed the Mac OS X executable of
the new XDS Viewer program to inspect diffraction images. I have moved
the program to /Applications. It starts up fine, but when I try to
load an image it complains:
"Cannot open file! For image formats other than ".c
Bram,
If you use the beta-test autotracing SHELXE, the .pdb file containing
the poly-Ala trace also contains the space group. You presumably meant
that the space group is missing from the SHELXL and SHELXD .pdb files,
but of course you can read the .res files into Coot.
George
Prof. George M. S
Hello Folks,
My enquiry pertains to membrane proteins. If you have been able to
successfully express a eukaryotic integral MEMBRANE protein in E.
coli or know of such a case, would you please provide some details.
Thanks.
Raji
A postdoctoral position is available for an experienced molecular
biologist/macromolecular crystallographer in the laboratory of Elisabeth
Sauer-Eriksson at Umeå University. The project involves
structure-function studies of proteins and protein complexes involved in
bacteria host cell interact
Dear All
for those of you who have ccp4 6.1.1 there is refmac 5.5.0088 on the
prerelease page
http://www.ccp4.ac.uk/prerelease/
plus on
ftp://ftp.ccp4.ac.uk/ccp4/6.1.1/updates
various bug fixes and tag.gz files of bug fixes and the refmac
updates for linux and os x
binary distributions
Dear all,
Graeme Winter wrote:
I find this very helpful for inspecting and using maps from shelxe.
There is no need to convert the phs to mtz if you just want to inspect
the map from Shelxe.
You can read in the .phs file into Coot. Since the .phs file does not
have unit cell dimensions, you
Hi Folks,
There is also convert2mtz which can be found in more recent CCP4
distributions. If you type convert2mtz it will give you details on
what is needed.
A couple of caveats with this:
- you need to quite the cell constants i.e. 'a b c alpha beta gamma'
- the spacegroup has to be written c
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