Re: [ccp4bb] Problem with multiple users running ccp4i on one machine

2009-03-22 Thread Miguel Ortiz Lombardia
Dear ccp4i developers, Jumping into this issue a bit late... I have some criticism to make here, please take them friendly, I make them precisely because ccp4i is so useful. I presume there are good reasons behind the new design of CCP4i and its CCP4 DBhandler, but I think you have overlook

[ccp4bb] Plasmids and other material [Was: images]

2009-03-22 Thread Miguel Ortiz Lombardia
Hi, I mostly agree with Artem, except on one point: As long as people publish most of the details necessary to reproduce the materials (protein samples and crystals) used in structure determination - the crystals may be reproduced 'by persons skilled in the art'. There is no need to even s

[ccp4bb] Interaction between the domains

2009-03-22 Thread peter hudson
Hello all I have a very quick question. Is there any programme, which can calculate the H-bond pattern or residues involved in H-bonds formation between the interface of monomers or the between the domains of the same monomer. I will appreciate your suggesstions. Thanks in advance Peter

Re: [ccp4bb] Plasmids and other material [Was: images]

2009-03-22 Thread Artem Evdokimov
Hi Miguel, I was deliberately extreme, in order to make my point (which is that the only item that *really* needs to be stored is information). Yes, it is in general cheaper to have plasmid samples stored on DNA cards. Total cost would be a few dollars depending on whether you use commercial DNA

[ccp4bb] Crick-Magdoff and anomalous

2009-03-22 Thread James Holton
I have had several people now ask me where the heck my "Crick-Magdoff" equation came from. So it looks like brevity has once again conquered clarity. So, those of you you enjoy my long emails read on. Those of you who do not, I direct you to the "Delete" button that should have come with you

Re: [ccp4bb] Purification

2009-03-22 Thread Kn Ly
Hello everyone, Just want to say thanks for your great ideas and time to reply my question. Hope I will solve my problem soon Kien