I recently had some success in this area. My approach was to get lucky
when I guessed the linker. In this case, my linker became ordered
which seemed to help my fusion construct settle down enough to exhibit
density. My linker sequence came was (approximately) alternating
hydrophilic and
Dear ccp4bb,
Thanks for the help. To summarise:
1) CCP4's tcl/tk installs into /usr/local/X11/bin
2) To point imosflm to this version of Tcl/Tk the line export
MOSFLM_WISH=/usr/local/X11/bin/wish8.4 needs to be in a file
called .bash_profile NOT .profile for some reason (but not if you are
Matt,
Your best bet would be to purchase the actual well condition from the
manufacturer. I know that both Hampton and Emerald can sell you a
larger quantity of what comes premixed in the screen. I am sure other
manufacturers can do the same. If you are lucky they might be able to
match the
Hi,
I have a protein-DNA complex grown in 30 -40% MPD/0.2M Ammonium sulfate/5% PEG
400/ 0.1M Tris (8.5).
I believe that 30%MPD above does not require any cryoprotectant but i hv a
problem to loop a crystal up. The crystal always sticks with AmSO4.
I tried to add 2ul up to 6ul
One way to do that is lower the concentration of AS form 0.2 to 0 or as
low as you can . This is depended on the xtal's stability . You can
transfer them directly or step by step.
Otherwise you can try to add water around the drop to increase the
humility or cover the drop by oil.
Good luck!
If you want to get rid of mother liquid around crystal, mount it in oil.
Howerver, what is wrong with NH4SO4? If your crystal can tolerate
near-saturated NH4SO4, it is a cryoprotectant all by itself.
-James Holton
MAD Scientist
HanJie_HCT Tai wrote:
Hi,
I have a protein-DNA complex
On Apr 3, 2009, at 3:05 AM, Simon Kolstoe wrote:
2) To point imosflm to this version of Tcl/Tk the line export
MOSFLM_WISH=/usr/local/X11/bin/wish8.4 needs to be in a file
called .bash_profile NOT .profile for some reason (but not if you
are using an X terminal in which case you need to
Postdoctoral position at the MRC Laboratory of Molecular Biology,
Cambridge, UK (closing date April 17, 2009)
Dr Murray Stewart is seeking a postdoctoral researcher to work on the
structure and function of macromolecules involved in nuclear transport
and cell division. The Stewart group
In the drop, the amSO4 becomes/casuses precipitate (I guess). The crystals were
grown from the precipitate within a few days. When I looped up the crystal the
precipitate was also looped up.
And I don't know whether AmSO4 is a cryoprotectant or not. When I freeze the
crystal in LN, the
I would think 30% MPD or more should normally be
enough to cryoprotect. If that is not the case, then you can always
supplement with additional MPD, glycerol, or glucose. If I understand
correctly, your crystals are forming in the presence of some additional
protein precipitate? If so, you
Dear All,
assuming the chiral volume definition with r() a Cartesian position vector
Vc = [r(N)-r(Ca)]*([r(C)-r(Ca)]x[r(Cb)-r(Ca)])
I always get about -0.65 A**3
Is this correct? Are there any target values?
In a SHELX manual I read + 2.5 for the chiral volume - seems to
be different.
(not
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