Re: [ccp4bb] hydrogen bond between aspartate side chain and main chain carbonyl group?

2009-04-27 Thread Lari Lehtiö
Hi, Not my structure, but noticed similar bond in glycerol dehydratase, when comparing structures. PMID: 16414072 pdb 1R9E and resid 506. (resolution 2.4 Å) Buried rather hydrophobic environment and Asp make H-bonds to serine hydroxyl and carbonyl. ~L~

Re: [ccp4bb] Twinning or not?

2009-04-27 Thread Eleanor Dodson
You are right ; extremely accurate NCS parallel to a symmetry axis does distort the plots, but its effect is usually resolution dependent so expected straight lines are often not straight.. And pseudo NCS translation can be even more confusing. Andrei Lebedev has done a lot of analysis on

Re: [ccp4bb] Watertidy troubles

2009-04-27 Thread Andrii Ishchenko
You can do this using coot. Just switch on showing symmetry mates(menu Draw-CellSymmetry). Then save the needed by Save Symmetry Coordinates Option in menu File. Waters coordinates will be saved as well. Regards, Andrii

[ccp4bb] Postdoctoral position available: Protein recognition of anesthetics

2009-04-27 Thread Patrick Loll
A postdoctoral position will be available Fall 2009 in Philadelphia, PA, USA. The successful candidate will join a collaborative research effort between the Eckenhoff laboratory at the University of Pennsylvania and the Loll laboratory at the Drexel University College of Medicine. This

[ccp4bb] Protein visualization screensaver released

2009-04-27 Thread Benjamin Blonder
Hi all, I know there was some interest in months past pertaining to a screensaver that would display PDBs. I've just updated my program for Mac OS X and am pleased to announce its release. Structure 2.0 is Intel compatible and is much faster and prettier than the previous version. You can

Re: [ccp4bb] Reason for Neglected X-ray Fluorescence

2009-04-27 Thread Jacob Keller
Quoting Jacob Keller j-kell...@md.northwestern.edu: Also, in your selenium crystal example, I think there would still be an anomalous signal, because there would always be regular scattering as well as the anomalous effect. Isn't that true? It is certainly not correct to state that there

[ccp4bb] [ccp4b] Removing Crystals from Automated Sitting Drop Trays

2009-04-27 Thread Matthew Alan Bratkowski
Hi. I was wondering if anyone had advice about removing crystals from 96 well, 3 drop sitting drop trays used for automated crystal screening. Recently, I was trying to remove crystals from an Intelli-plate designed by Art Robbins Instruments, which has three small drop wells next to a

Re: [ccp4bb] [ccp4b] Removing Crystals from Automated Sitting Drop Trays

2009-04-27 Thread Artem Evdokimov
Yes :) It's a common issue that requires a modicum of planning and dexterity to overcome: * cutting is done under a macroscope - it's much easier to see what you're doing * firm pressure on the plate (with one hand grasping the sides) is key to success * firm downward pressure of the