[ccp4bb] Postdoctoral Position Available in Structural Biology/Biophysics in Maryland, USA

2009-05-15 Thread Scott Walsh
A NIH funded postdoctoral position is available at the Center for the Advanced Research in Biotechnology (CARB) at the University of Maryland Biotechnology Institute in Rockville, Maryland, USA. Several research projects are available to study the interleukin-7 signaling pathway at the struc

[ccp4bb] ESRF Rolling access beamtime

2009-05-15 Thread David FLOT
Dear all, The ESRF is pleased to remind you about the procedure for applying for rolling access beamtime on its MX beamlines. The rolling access procedure is designed to improve access to ESRF MX beam-lines and encourage the use of ESRF facilities by smaller groups working in the MX field. Thi

Re: [ccp4bb] Experimental design and response surface methods for crystal optimization

2009-05-15 Thread Charles W. Carter Jr.
Christian, I happened to see your post to the CCP4 bulletin board. In answer to your question, we continue to base all of our work on proper experimental design. Two papers cited below document the flexibility and power of response surface methods in biochemical experimentation, especiall

[ccp4bb] Experimental design and response surface methods for crystal optimization

2009-05-15 Thread Benda, Christian
Dear all I recently came across the "experimental design" method for systematic optimization of multiparametric problems like macromolecular crystal growth. (The response surface concept has been introduced to protein crystallization by Carter et al. (e.g. Meth. Mol. Biol, vol. 363) and should t

Re: [ccp4bb] problem with unsorted mtz files

2009-05-15 Thread Phil Evans
It might be worth combining these files in Pointless before Scala to check that they are compatible Phil On 15 May 2009, at 14:28, Rita GIORDANO wrote: Dear all, I have 8 data set from insulin at 1.2 A, after used Mosflm and Scala I used tha program Sortmtz to reset the batch number, first

[ccp4bb] problem with unsorted mtz files

2009-05-15 Thread Rita GIORDANO
Dear all, I have 8 data set from insulin at 1.2 A, after used Mosflm and Scala I used tha program Sortmtz to reset the batch number, first, and after unsorted MTZ files in one mtz file. When I put this mtz file in SCALA I have the follow error message: Insufficient data to determine param

Re: [ccp4bb] Phasing at Low Resolution

2009-05-15 Thread bas
Dear Clemens, sounds like typical pathology of plate-like crystals, which often suffer from slight growth defects and possibly deformation during handling/freezing. If the Se-Met crystals are indeed easy to obtain in large numbers you may want to test as many of them as you can, because there can

Re: [ccp4bb] Na/K Phosphate

2009-05-15 Thread Shveta Bisht
Hi Sodium phosphate monobasic has higher solubility than sodium phosphate dibasic, while it is other way round for potassium phosphate salts. Potassium phosphate dibasic is more soluble than potassium phosphate monobasic. So the best choice is to make solutions of sodium phosphate monobasic and pot

[ccp4bb] Protein Purification Research Associate position

2009-05-15 Thread Felicetti, Brunella
Scientist/Senior Scientist position Protein purification, Evotec, Abingdon, UK Applications are sought for a Scientist/Senior Scientist position in the Protein Production team at Evotec Job Description: Based at Abingdon, Oxfordshire, Evotec offer integrated services which cover the entir

Re: [ccp4bb] Phasing at Low Resolution

2009-05-15 Thread Clemens Vonrhein
On Thu, May 14, 2009 at 03:03:57PM -0400, Guangyu Zhu wrote: > Zn and Se have similar f' and f''. Why Zn seems have much better phasing > powder in practice? Is it just because Zn always binds tightly on protein > but Se might have higher b-factor? Also: Met sidechains often adopt alternate confor

[ccp4bb] Beamtime proposal deadline for ALS GU time

2009-05-15 Thread Peter Zwart
May 15, 2009 is the deadline for the July-August Rapid Access Proposal cycle. Please note that the ALS will be shut down in September, 2009. The deadline for applications of beamtime on the BCSB beamlines in the ALS is today! Other notable features include: * A high-accuracy microdiffractom