Re: [ccp4bb] Phasing at Low Resolution

2009-05-15 Thread Clemens Vonrhein
On Thu, May 14, 2009 at 03:03:57PM -0400, Guangyu Zhu wrote: Zn and Se have similar f' and f''. Why Zn seems have much better phasing powder in practice? Is it just because Zn always binds tightly on protein but Se might have higher b-factor? Also: Met sidechains often adopt alternate

Re: [ccp4bb] Phasing at Low Resolution

2009-05-15 Thread bas
Dear Clemens, sounds like typical pathology of plate-like crystals, which often suffer from slight growth defects and possibly deformation during handling/freezing. If the Se-Met crystals are indeed easy to obtain in large numbers you may want to test as many of them as you can, because there

[ccp4bb] problem with unsorted mtz files

2009-05-15 Thread Rita GIORDANO
Dear all, I have 8 data set from insulin at 1.2 A, after used Mosflm and Scala I used tha program Sortmtz to reset the batch number, first, and after unsorted MTZ files in one mtz file. When I put this mtz file in SCALA I have the follow error message: Insufficient data to determine

Re: [ccp4bb] Experimental design and response surface methods for crystal optimization

2009-05-15 Thread Charles W. Carter Jr.
Christian, I happened to see your post to the CCP4 bulletin board. In answer to your question, we continue to base all of our work on proper experimental design. Two papers cited below document the flexibility and power of response surface methods in biochemical experimentation,

[ccp4bb] ESRF Rolling access beamtime

2009-05-15 Thread David FLOT
Dear all, The ESRF is pleased to remind you about the procedure for applying for rolling access beamtime on its MX beamlines. The rolling access procedure is designed to improve access to ESRF MX beam-lines and encourage the use of ESRF facilities by smaller groups working in the MX field.

[ccp4bb] Postdoctoral Position Available in Structural Biology/Biophysics in Maryland, USA

2009-05-15 Thread Scott Walsh
A NIH funded postdoctoral position is available at the Center for the Advanced Research in Biotechnology (CARB) at the University of Maryland Biotechnology Institute in Rockville, Maryland, USA. Several research projects are available to study the interleukin-7 signaling pathway at the