The answer to this was to ensure that the file
/etc/hosts
is present and contains the line
127.0.0.1 localhost
William G. Scott
Contact info:
http://chemistry.ucsc.edu/~wgscott/
On May 16, 2009, at 6:38 AM, Sebastiano Pasqualato wrote:
hi there,
I'm using ccp4 installed via fink
Try things that affect nucleation and kinetics of crystallization,
such as seeding or crystallization at different temperatures. As
Morten suggested, try different crystallization methods such as
switching between hanging/sitting drop and microbatch. Screen protein
concentration and protein:crystal
Dear Heng,
I assume you are growing your crystals using vapor diffusion. If your
optimization efforts using various additives fail to give you suitable
crystals you might consider trying to grow your crystals using a different
technique. I would suggest a liquid diffusion based technique such
Hi,
I have a 22kDa protein that the floopy N & C terminus have been deleted. It was
crystallized in 35%MPD/0.1m Tris (pH 8.5)/0.2M/(NH4)2SO4 in 2 - 3 days.
Previously, a few small twining crystals were grown in this condition.
I tried Hampton Research 96-additive screen . Additives such
