Re: [ccp4bb] low UV reading on AKTA prime

2009-07-01 Thread Lijun Liu
Hi Matt, AKTA system does allow you check the how-good percentage of the UV lamp, so if the UV lamp is new, and it gives low percentage (I do not mean the 20%) then your AKTA system may have the problem like Scott said or other thing in the Optical path. There are other possibilities, however! F

Re: [ccp4bb] multi-domain protein with identical tertiary structure

2009-07-01 Thread David Briggs
Hi Shankar, If you mean what I think you mean - yes, hundreds - but it depends on how strict you are about "identical' Lots of proteins have repeat domains in them with the same fold (and homologous sequence) and therefore very similar (if not quite identical) tertiary structure. Some examples i

[ccp4bb] multi-domain protein with identical tertiary structure

2009-07-01 Thread Shankar Prasad Kanaujia
Dear CCP4 users, Is there any multi-domain protein (with at least two domains) which has identical tertiary structure of each domain ? Thanking you. -regards shankar

[ccp4bb] repeated spamming of list members

2009-07-01 Thread William G. Scott
I've been getting multiple spams with subject line "Offer microorganisms for investigation" along with others who are on the ccp4bb list. The sender has a gmail account. You can complain here if you are getting these too: http://mail.google.com/support/bin/request.py?contact_type=abuse_phis

Re: [ccp4bb] space group R32: H settings

2009-07-01 Thread George M. Sheldrick
Why are you running shelxc/d/e from ccp4? If you run them directly from a short script or using the hkl2map GUI from Thomas Schneider, there is no problem reading such a .sca file correctly (I have just checked it with another structure in the same space group). Since there is some confusion abo

[ccp4bb] space group R32: H settings

2009-07-01 Thread Jerry McCully
Dear All: I think this is an old topic. But I could not find the previous discussion about it. Therefore, I am asking for your help again here. My data were processed as primitive rhombohedral R32 using HKL2000. If I am not wrong, each (h, k, l ) in the .sca file wa

Re: [ccp4bb] low UV reading on AKTA prime

2009-07-01 Thread Patrick Loll
I second Scott's post. About the only problem we've had with our Akta instruments is this type of degradation of the filter. I'm not sure what the mechanism is, but the filters do seem to crap out after a while, at least in the cold room (oxidation? I have no idea of what the filter is mad

Re: [ccp4bb] low UV reading on AKTA prime

2009-07-01 Thread Scott Walsh
Hi Matt, Check to make sure the A280 filter is clean.  Ours was filthy.  You might need to replace this (~$350).  We were experiencing the same thing you mentioned and just had the GE technician out today.  Also, make sure the A280 dial is set correctly on the lamp.  Please check the manual for

[ccp4bb] low UV reading on AKTA prime

2009-07-01 Thread Matt Colins
Hi all, We recently got low UV reading on our AKTA prime. We contacted GE healthcare, but was told that the UV reading on AKTA prime should be 20% of the spectrometer reading, because the path length of the flow cell of AKTA prime is only 2 mm (20% of the pathlength of a spectrometer cuvette).

Re: [ccp4bb] dial box

2009-07-01 Thread David Roberts
I have a few of these if people are interested. Please just let me know off list. The ones I have are the 8 dial boxes connected to an old SGI workstation (they work in linux as well I believe - with O). I simply don't use these anymore and wouldn't mind parting with them. Thanks Dave >>>

Re: [ccp4bb] Best 3D stereo combination for Linux

2009-07-01 Thread Warren DeLano
David, > Warren: why should a 120 Hz LCD monitor with the nVidia USB > emitter/glasses require a Quadro card? The system requirements for > GeForce 3D Vision on Windows lists many GeForce cards as being > supported: Because if you dig a bit deeper, you'll discover that GeForce cards only support

[ccp4bb] dial box

2009-07-01 Thread Tobias Krojer
Dear all, does someone still have a couple of functional dial boxes and wants to sell them? I guess one cannot buy them anymore?! Thanks, Tobias

Re: [ccp4bb] Best 3D stereo combination for Linux

2009-07-01 Thread David J. Schuller
Warren: why should a 120 Hz LCD monitor with the nVidia USB emitter/glasses require a Quadro card? The system requirements for GeForce 3D Vision on Windows lists many GeForce cards as being supported: http://www.nvidia.com/object/GeForce_3D_Vision_Requirements.html GeForce GTX 200 series ... Gef

[ccp4bb] Best 3D stereo combination for Linux

2009-07-01 Thread Phoebe Rice
Sorry this has probably come up already, but we're about to become desperate for a new stereo-capable machine, to be attached to an existing linux server. Is there a currently proven-to-work LCD stereo system for linux that works with Coot and/or O as well as pymol? Or should we recycle the aging

Re: [ccp4bb] Best 3D stereo combination for Mac OS X today

2009-07-01 Thread Warren DeLano
Danny, Thank you (and Harry and Dirk) for pointing out the after-market PNY card -- I wasn't aware of that option. However, it is worth noting that PC users can access stereo 3D (via "3D Vision" IR emitter & glasses) with a $139 Quadro 300-series card whereas Mac users must spend 12X more fo

Re: [ccp4bb] Best 3D stereo combination for Mac OS X today

2009-07-01 Thread Dirk Kostrewa
Dear Warren and Danny, I was also surprised that the configuration of the new Mac Pros doesn't offer a Quadro graphics card anymore. However, Apple still sells an NVIDIA Quadro 4800 for Mac. Best regards, Dirk. Am 01.07.2009 um 00:25 schrieb Warren DeLano: Danny, Last I checked, Apple