Hi Engin,
Two thoughts:
* Is the Patterson map consistent ? (with different resolution ranges,
exclusions, ...). You could possibly give 'GraphEnt' a try [assuming it
still works with current generation mtz files].
* The number of heavy atoms may be much larger and may deviate from
the know
There is a program called FSEARCH in the CCP4 Suite that will dock EM
maps, SAXS envelopes or what have you into a unit cell.
However, I am not aware of any instances where a novel crystal structure
(~4 A or better and no NCS) was solved this way. It is not for want of
trying! I think the la
Hi Francis,
There is an older paper that mentions this idea: Tsao et al, Acta Cryst B48
(1992), 75-88. However, when you look at the paper, small-angle scattering data
is not the only thing that was used. In particular, if my memory serves me
right, the 60-fold averaging applied to the proble
You can use the glass cell disruption pestle
http://www.vwrsp.com/catjpg/mp0/mp0440.jpg
to grind them up. They cost money, of course, and if you plan to buy
something, why not go for seed bead kit? But if you want something very
cheap, then you'll find this amazing discovery interesting:
"Oute
Neha Kharbanda wrote:
Dear All,
I submitted my processed.hkl file to the Merohedral Twin server and
the results are bit strange the curve corresponding to my data
goes below the perfectly untwinned red line..the complete opposite of
the theoretically twinned red curve line.
Has anyone
Hi all
I'm looking for anyone who has had (practical) experience using SAXS
data to phase 4.2 A crystals. Please email me.
FR
-
Francis Reyes M.Sc.
215 UCB
University of Colorado at Boulder
gpg --keyserver pgp.mit.edu --recv-keys 67BA8D5D
8AE2 F
Dear All,
I submitted my processed.hkl file to the Merohedral Twin server and the
results are bit strange the curve corresponding to my data goes
below the perfectly untwinned red line..the complete opposite of the
theoretically twinned red curve line.
Has anyone seen such a thing befor
Joint Autumn Meeting of the IG (Industrial Group) and YCG (Young
Crystallographers Group), 2009
At The World of Glass, St. Helens
The meeting will be held on Thursday 5th November 2009
Runs from 10.30 till 16.30
With free registration for all YC's
For non-YC members the registration fee is £8
The problem:
Extremely low Bfactor values for water molecules after TLS refinement
(Bfact. = 2) despite being associated with sidechains with much higher
Bfactors. The problem apparently arises because REFMAC includes the waters
in TLS groups by default.
The solution:
exlude the waters from TLS re
Dear all
Anybody has experience in protein crystallization at pH 2.0? Conditions,
buffers, temperature...?
Thanks a lot
Marta
Dr. Marta Martínez Júlvez
Departamento de Bioquímica y Biología Molecular y Celular
Facultad de Ciencias
Universidad de Zaragoza
Pedro Cerbuna 12
50009-Zaragoza
Spain
Yes - I have had a similar problem..
We need a reply from Garib..
Eleanor
Alasdair K. Mackenzie wrote:
Greetings CCP4-ers,
I have been running some TLS refinement which does wonders for my R/Rfree
values, but seems to give some spurious Bfactors/ADPs for the waters, e.g.
B= 2.
These low Bfa
Dear All,
A permanent post is on offer at Syngenta. Enquiries are welcome but please only
apply online via the website.
Protein Crystallographer
Jealott's Hill, Bracknell, Berkshire
Syngenta is one of the world's leading companies with more than 24,000
employees in over 90 countries. As
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