Re: [ccp4bb] scala maxbat compile problems

jpd, I have seen this type of error message in situations where some memory limit was exceeded. Based on googling with 'common relocation truncated to fit ', the memory limit has something to do with a COMMON block exceeding its max size, see -

Re: [ccp4bb] pdbcur failed with the error message 'child process exited abnormally'

The big problem here is that it doesn't recognise the NOANISOU keyword. So I presume that you are picking up an old version of CCP4, or more likely a Coot version of pdbcur. Mmmm the fact that the banner doesn't report user or date or time suggests a dodgy version. I would have to defer to a Mac

[ccp4bb] To get the crystal faster...

Dear crystallographers, Sorry for the non-ccp4 query. I am new to this field and need some suggestions. My question is, why some protein takes longer time to crystallize, say 6-8 months, and it is the only condition to get the crystals.? What are the ways to get the crystals faster. The crystal

Re: [ccp4bb] To get the crystal faster...

It will help more if we send it to James ;) On Oct 11, 2009, at 9:15 AM, gauri misra wrote: Dear James, As there are indications of protein degradation that have been suggested in previous postings, i think adding some protease inhibitors right at the stage of purification may provide

Re: [ccp4bb] To get the crystal faster...

Hi James, have you tried limited proteolysis on your protein and see if you can identify a stable fragment. Then re-clone and re-crystallize your protein. Or a very stupid suggestion, how does your size exclusion peak look like ? What you're not running your protein over a SEC to polish

[ccp4bb] To set a crystal tray with protein and Trypsin protease.

Hello, I am sorry to put this question which didn¹t related to the CCP4 software. I couldn¹t get the crystals from the protein only. So I want to try the different way to work it out. I hear that it is possible to set a crystal tray of protein with Trypsin protease. I will be very appreciated

[ccp4bb] problems with molprobity in coot

Hi everyone, I am trying to use molprobity to check a structure in coot (platform is 64-bit vista). I followed the instructions found here to set up molprobity: http://www.ysbl.york.ac.uk/~lohkamp/coot/wincoot-faq.html . From the logs, it seems like reduce runs OK (it says that it Added 6076

Re: [ccp4bb] To get the crystal faster...

Sometimes it is better that it takes time for crystals to appear. Remember that crystallisation is a purification procedure. A way to decrease the speed of crystallisation is to use Dunlop's and Haze's drop dilution method (K. V. Dunlop B. Hazes (2003). When less is more: a more efficient

Re: [ccp4bb] To set a crystal tray with protein and Trypsin protease.

Hi Jing, Methods to perform In site proteolysis are available in the following publications: http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0005094 http://www.nature.com/nmeth/journal/v4/n12/abs/nmeth1118.html Good Luck, -Partha On Sun, Oct 11, 2009 at 5:13 PM,

Re: [ccp4bb] To set a crystal tray with protein and Trypsin protease.

It should be In situ and not 'In site'. Sorry for the typo error. -Partha On Sun, Oct 11, 2009 at 9:59 PM, Parthasarathy Sampathkumar spart...@gmail.com wrote: Hi Jing, Methods to perform In site proteolysis are available in the following publications: