Apologies for an off topic question. I was hoping for some advice regarding
the use of sulfo-NHS diazirine crosslinker (Pierce
linkhttp://www.piercenet.com/products/Browse.cfm?fldID=DB013E61-5056-8A76-4EC4-473A6F2468A1)
for in vitro protein interaction analysis.
Have tried labeling the protein
Hello,
I am working with refmac-nm code derived from refmac5
and I am encountering an error below
[...]
Open failed: Unit: 7, File: /tmp/refmac-nm_temp1.21850_new.crd (logical:
/tmp/refmac-nm_temp1.21850_new.crd)
BFONT COLOR=#FF!--SUMMARY_BEGIN--
Refmac_5.2.0019: Open failed:
Dear all,
we have seen again with the 5.5.0102 version that water O or Fe ion
are not held on the crystallographic axis automatically.
What should we do except using possibly the restrain keyword?
Jan Dohnalek
--
Jan Dohnalek, Ph.D
Institute of Macromolecular Chemistry
Academy of Sciences of
Apologies for an off topic question. I was hoping for some advice regarding
the use of sulfo-NHS diazirine crosslinker (Pierce
linkhttp://www.piercenet.com/products/Browse.cfm?fldID=DB013E61-5056-8A76-4EC4-473A6F2468A1)
for in vitro protein interaction analysis.
Have tried labeling the protein
Apologies for an off topic question. I was hoping for some advice regarding
the use of sulfo-NHS diazirine crosslinker (Pierce -
http://www.piercenet.com/products/Browse.cfm?fldID=DB013E61-5056-8A76-4EC4-473A6F2468A1)
for in vitro
protein interaction analysis.
Have tried labeling the protein
Hi all,
I'm looking at getting some nickel magnetic beads. I've never used them
before, and they seem pretty expensive. Does anyone know of a good inexpensive
supplier?
Thanks in advance for any replies!
Hello everyone,
Sorry for a non-CCP4 related question again. Can anyone let me know how to
make stereo work on linux with Zalman monitor with Coot? Is it as simple as
what we do with CRT monitors? Or do we need something else? We presently use
CRT monitors on a Quadro FX 4600 graphics
Hi Ajit;
One of our CRT monitors broke recently, and in the context of
bemoaning the loss to a friend I was told that LCD monitors will not
work for stereo viewing. I understood the reason to be related to the
difference in refresh rates (?), with LCD's not being fast enough so
that the
Rubbish, excuse my English :-)
I have four Zalman's running in stereo although I have to confess
they're all running on various Macs (MacBook Pro, Mac Mini, MacPro)
under 10.5 or 10.6.
I have no clue if the Zalman runs under linux.
Regarding the stereo effect it is superior than conventional
The Zalman defeats these with a very smart trick. We got out first
one, and it's really very good, albeit not perfect in some details.
Pymol and Coot worked just like that with it.
Sent from my iPhone
On 4 Nov 2009, at 19:26, Justin Hall hallj...@onid.orst.edu wrote:
Hi Ajit;
One of our
I can personally verify that the new Nvidia 3D Vision system on 120 Hz LCD
monitors and a Nvidia Quadro FX Video Card display stereo flawlessly in
Pymol, Wincoot, UCSD Chimerica, and any other application that uses OpenGL
or Direct3D as its display media within both Windows Vista and Windows 7
Thanks to everyone for the info on Zalman monitors, sorry to have
muddied the waters for you Ajit. Best wishes~
~Justin
Quoting Justin Hall hallj...@onid.orst.edu:
Hi Ajit;
One of our CRT monitors broke recently, and in the context of
bemoaning the loss to a friend I was told that LCD
I should add that I have only seen Joel Bard's Zalman monitor briefly.
We tweaked the settings a bit to improve the representation in the
following way:
1) use nvidia-settings to override application anti-aliasing - crank it
up to 16x if you have a good graphics card
2) add to ~/.coot file
Dear All,
as a first step towards building the web resources for my book
Biomolecular Crystallography: Principles, Practice and
application to Structural Biology I have made publicly
available the Index and the Glossary in searchable PDF format.
I hope this might help locating contents more
Dear Friends,
I can create a mesh in pymol as follows:
isomesh 2ffmesh, nov42_001_2mFo-DFc.map, 2.5, sele, carve=1.6, where the 'sele'
is a particular residue and the map is created ommiting that residue from the
pdb.
But, I can't create the mesh in pymol when I am using Fo-Fc
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