I am sorry for being so offensive. I did not realize such a
consequence for my opinion or misunderstanding, which was not my
intention. I just want to say I feel really bad for such a tragedy.
Sorry again. Aidong
On Jan 10, 2010, at 8:28 PM, Anastassis Perrakis wrote:
Although he m
Dear all,
a solved 2.6 Ang. structure shows the following estimated coordinate errors
REMARK 3 ESTIMATED OVERALL COORDINATE ERROR.
REMARK 3 ESU BASED ON R VALUE(A): 0.468
REMARK 3 ESU BASED ON FREE R VALUE (A): 0.279
REMARK 3 ES
Dear All,
we perform refolding of our protein by diluting it 1:10 fold, but we get a
low yield in it. I have studied about pulsatile refolding in the book
"Therapeutic
proteins: methods and protocols''. Here it states use of peristatltic pump
at flow rate of 0.5 ml / min to add solubilization bu
Dear crystallographers,
Macromolecular X-ray crystallography core of Scripps FL offers equipment
and resources to scientists outside (academic and industrial) of our campus
by providing crystallographic analysis of their chosen biological
macromolecules. We offer full service by performing prote
Postdoctoral Program at MAX-lab
The MAX-laboratory is a Swedish National Laboratory in Lund, Sweden. It
presently consists of three electron storage rings. It provides photons from IR
up to the hard X-ray regime and serves 800 scientists a year. MAX-lab is in the
process of constructing a new f
My knowledge of Fortran is dated, but the ESU_R and ESU_RFREE are
calculated in refmac like this
HESU_CRUIC = SQRT(FLOAT(N_REFINED_ATOMS)/FLOAT(NREFMNPAR))*
+ COMPL**(-0.33)*HD_HIGH*HRFAC_SHELL_WORK(NB1)
HESU_FREE = SQRT(FLOAT(N_REFINED_ATOMS)/FLOAT(NREFA(1,NB1)))*
+
