Dear all CCP4 experts,
Hello, I'm not molecular biologist and quite new in this field. I would like to
know what companies (i.e. Sigma/Aldrich) are possibe for me to purchase
purified protein samples. Any suggestion is welcomed.
Many thanks in advance.
stephen
--
Dr. Stephen Sin-Yin Chui
Dear all,
can anyone share experience with measuring membrane protein
concentration in detergent containing buffer with a nanodrop photometer
e.g. Thermo Scientific ND2000. Specifically, does the reduction in
surface tension caused by the detergent pose any problems?
Thanks!
Cheers
Florian
Dear all,
I am looking for the sugar-validation tool pdb-care -- the
glycosciences.de website seems to be down, sadly.
Are pdb-care and the other rather valuable glycosciences tools available
somewhere else? I'd prefer to run them locally -- are they available as
stand-alone applications?
Hi Judit,
Thomas Lütteke developed pdb-care and several of the other glycosciences.de
tools. Although he has since moved on, I understand he still devotes time to
maintaining these services.
Last known address t.tuetteke AT chem.uu.nl
All the best,
Tim
-Original Message-
From:
Hi,
I was in touch with Thomas Luetteke recently about this, and he is hoping to
move the server to his current location in Giessen, probably around February.
He says that it would be difficult, at least at the moment, to install locally.
Regards,
Randy Read
On 25 Jan 2010, at 12:06, Tim
Hello Florian,
We routinely measure membrane protein samples in detergent with much problem
on the nanodrop. The concentration of detergent is often many times the CMC
. We have found the drop does form quite well as long as the surface is
clean.
Often this can be easily achieved by repeated
Dear all,
I am trying to solve a structure at 2.05 A resolution by molecular replacement.
The space group
seems to be P21, with unit cell dimension 52.63, 29.43, 104.970 and beta =
95.60.
Only one copy of the protein should be present in the asymmetric unit, with 58%
of solvent content.
The
Hi CCP4ers
As requested I have compiled the list of responses I got regarding anions in
protein structures. I did not receive a huge number (wrong time of day
perhaps..) but I do think that the list below covers the various aspects
(specific examples as well as various studies) and was very
Hello Stephen,
Of course it depends on what proteins you want. You can buy some
easily crystallizable proteins from Hampton Research. Some purified
proteins (proteases, lysozyme, calmodulin, etc) can be purchased from
Sigma. Or are you looking for custom expression and purification?
ho
We are offering RapiData 2010, the twelfth offering of our popular course:
Rapid Data Collection and Structure Solving at the NSLS: A Practical
Course in Macromolecular X-Ray Diffraction Measurement
The course will be held 11-16 April 2010. Students could be at any level from
Dear all,
Brief Summary:
BioKlatch (http://www.BioKlatch.com) is a new web forum meant to
supplement mailing lists. It uses software that's popular with
mathematicians and software developers on http://mathoverflow.com and
http://stackoverflow.com. A post can be given multiple tags such as
At the risk of creating another runaway thread, I have spent some time
trying to reconcile what Ian was talking about and what I was talking
about. The discussion actually is still relevant to the original posted
question about refining against images, so I am continuing it here.
Ian made a
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