[ccp4bb] Two Research fellowship positions

2010-08-10 Thread James Whisstock
Hi all We are seeking one or two postdoctoral researchers with a demonstrated expertise in biochemistry and protein crystallography and an interest in the structure and function of pore forming proteins and membrane proteins. Each fellowship includes a consumables budget as well as funding for

Re: [ccp4bb] To view the 2mFo-DFc map generated by SIGMAA

2010-08-10 Thread Hailiang Zhang
Dear Tim: This is also what I thought. Thanks! Best Regards, Hailiang > Dear Hailiang, > > the man-page of sigmaa claims > FWT (2m|Fo| - D|Fc|) exp(i AlphaCalc) > Analogous to 2Fo-Fc map, FFT input: F1=FWT PHI=PHIC >

Re: [ccp4bb] To view the 2mFo-DFc map generated by SIGMAA

2010-08-10 Thread Tim Gruene
Dear Hailiang, the man-page of sigmaa claims FWT (2m|Fo| - D|Fc|) exp(i AlphaCalc) Analogous to 2Fo-Fc map, FFT input: F1=FWT PHI=PHIC ^^ so I would leave out WCMB. Tim On Tue, Aug 10, 2010

Re: [ccp4bb] anisotropic Bs

2010-08-10 Thread Ethan Merritt
On Tuesday 10 August 2010 10:30:17 am Sue Roberts wrote: > Hello Everyone > > There's probably an easy way to do this, but I haven't found it. > > I've refined a 1.1 A structure with refmac and want to inspect the thermal > ellipsoids. Specifically I want to know if any of them are non-positive

[ccp4bb] To view the 2mFo-DFc map generated by SIGMAA

2010-08-10 Thread Hailiang Zhang
Hi there: When I generate the mtz file by SIGMAA, and want to view the 2mFo-DFc map in coot, should I choose "FWT PHIC WCMB" combination or just "FWT PHIC"? I think the later is more reasonable and I did see somebody the former as well. Didn't see explicit instruction in SIGMAA document, and I app

[ccp4bb] anisotropic Bs

2010-08-10 Thread Sue Roberts
Hello Everyone There's probably an easy way to do this, but I haven't found it. I've refined a 1.1 A structure with refmac and want to inspect the thermal ellipsoids. Specifically I want to know if any of them are non-positive definite and I want to know which have very large anisotropy. (I ca

[ccp4bb] [Fwd: [ccp4bb] Permanent X-ray Lab Manager Post London UK]

2010-08-10 Thread Nicholas Keep
There is just over a week left to apply for this X-Ray Lab Manager job in central London. Best wishes Nick -- Prof Nicholas H. Keep Executive Dean of School of Science Professor of Biomolecular Science Crystallography, Institute for Structural and Molecular Biology, Department of Biological Scie

Re: [ccp4bb] Stereo Mac OS X - Update

2010-08-10 Thread Tim Gruene
Zalman availability and price: The German retailer alternate claim the M215W is available and sell it for 339Euro (including VAT): http://www.alternate.de/html/solrSearch/toArticle.html?articleId=151410&query=zalman+&referer=detail&link=solrSearch%2Flisting.productDetails Cheers, Tim On Tue, A

[ccp4bb] Postdoctoral Fellowship in Structural Virology – Boston, MA

2010-08-10 Thread Katya Heldwein
A postdoctoral position is available to study proteins involved in herpesvirus entry, egress, and latency in the Heldwein lab at Tufts University School of Medicine, Boston, MA. Work will involve overexpression of membrane-bound and soluble proteins in eukaryotic cells followed by their crystalliz

Re: [ccp4bb] Stereo Mac OS X - Update

2010-08-10 Thread David Schuller
I can give general info about current stereo LCD solutions, but I don't know which ones work and/or are supported on Mac OS. The Zalman monitor may be scarce because it is being replaced by something newer; the 1680x1050 resolution has been replaced by 1920x1080. The specs you list appear mist

Re: [ccp4bb] Stereo Mac OS X - Update

2010-08-10 Thread mark Mayer
1) A couple of people like the Zalman BUT when I contacted the vendor recommended on the WIKI here's the response (edited). Note that the price has gone up, and its currently not available; my guess this is a US wide lack of availability? "We are totally sold out of these and Zalman is not prov

Re: [ccp4bb] cphasematch

2010-08-10 Thread Eleanor Dodson
Should work if you have the same indexing convention.. Another of Kevins utilities; csymmmatch -pdbin old.pdb -pdbin-ref SADbuild.pdb -origin-hand will compare the two models, and correct for symmetry and alternates due to reindexing I believe.. Eleanor wtempel wrote: Dear colleagues, her

Re: [ccp4bb] Does phenix have any utilities which can do B-factor sharpening?

2010-08-10 Thread Eleanor Dodson
Hailiang Zhang wrote: Hi there, Does phenix have any utilities which can do B-factor sharpening (with user-specified Bsharp values) when calculating maps? Thanks! Best Regards, Hailiang You know you can do this in coot on the fly, and test different Bs visually? Eleanor

Re: [ccp4bb] cphasematch

2010-08-10 Thread Phil Evans
You can check for consistent indexing using the program Pointless. The more recent versions (from version 1.5.0, perhaps best obtained from ftp://ftp.mrc-lmb.cam.ac.uk/pub/pre/) can use a PDB file as the reference (calculating structure factors from coordinates) Phil On 10 Aug 2010, at 06:58,