If you are afraid by the possible low incorporation of selenium in your
protein, and if you have some cysteines into your sequence, you can try the
double labeling --> Structure, Vol. 11, 1359–1367, November, 2003.
It works at the first try for me and I had no change in crystallization
conditions.
Persons of interest:
I understood that refinements in refmac of low res structures without
hydrogens includes a component of the Hydrogen atom attached to the
carbon in the carbon scattering factors. Or is this just if the Hydrogen atoms
are in the riding position. If so, why bother to add hydrog
Dont forget all the "atomic resolution" 3Å structures!
From: Pavel Afonine [pafon...@gmail.com]
Sent: Thursday, January 06, 2011 6:23 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Fwd: [ccp4bb] FW: [ccp4bb] Resolution and distance
accuracies
Hi,
creat
Dear Gerard
Yes I had seen this paper before but forgot about it (was probably in my
subconscious). Wladek also mentioned this to me and I was in the process of
acknowledging this when your email arrived.
The paper by Wladek and co-authors covers much (and more) of what has been said
on the bul
Hi,
creative language, you say... ha-ha. Go through this:
A number of publications define ultrahigh or subatomic resolution in the
range of 1.0–0.5A (Lecomte et al., 2008; Petrova et al., 2006; Howard et
al., 2004; Guillot et al., 2008; Housset et al., 2000).
Right? When you see ultra-high resolu
Dear Colin,
Wladek Minor has just drawn my attention to the following recent paper:
Acta Cryst. (2010). D66, 1041.1042
(that I must admit to having failed to notice) also expressing reservations
about some uses of "creative language".
With best wishes,
CCP4-o-philes,
Has anyone experienced issues with Phaser outputting P3 symmetry
MTZ and PDB files from H3 (R3) input files? I've worked on a
couple of structures in H3 in the last few months, and for some
reason Phaser is changing the symmetry of the input
Dear all,
I try to express selenomethinine-labeled protein in E.coli methinoine
auxotrophic strain B834 in M9 with 17 amino acid and 50 mg/ml se-met for 18
hours at 37 degree. I don't why the colour of medium vary from colorless to
yellow. Could anybody tell me it is normal or not?
Thanks a lot!
Maybe if you are really into this, you could "go public" by writing a
letter to the editor (from the CCP4BB?), and I am sure there will be a
good response from the authors...
Jacob
***
Jacob Pearson Keller
Northwestern University
Medical Scientist Training
I had good audio for the Buster talk. However, early during
Jeff Headd's talk, the audio level dropped to a level that
with maximum amplification could still not really be understood-
sort of like the wrong microphone was turned on. Later near the
end of the talk and during the question/answer se
Video is fine.. Anyone getting audio (or is it my realplayer
setup? ) ...
F
On Jan 6, 2011, at 10:04 AM, ronan.kee...@stfc.ac.uk wrote:
Dear all,
The web stream is now working and can be accessed from:
http://extrplay.dl.ac.uk/
There will be full coverage tomorrow (Friday). Apologies agai
Dear all,
The web stream is now working and can be accessed from:
http://extrplay.dl.ac.uk/
There will be full coverage tomorrow (Friday). Apologies again for the delay.
Best wishes,
Ronan
-Original Message-
From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of
rona
On Thu, 2011-01-06 at 11:30 +0800, Dr. STEPHEN SIN-YIN, CHUI wrote:
> how can I obtain a monomer library CIF (_lib.cif) of
> a new small molecule that could be recognized by Refmac5?
http://strucbio.biologie.uni-konstanz.de/ccp4wiki/index.php/Servers_for_ligand_topologies/parameters
--
"I'
I too think the phrase super-resolution is rather misleading, in particular the
analogy with light microscopy methods. Super-resolution in these latter cases
is achieved via different physical phenomena (think excitations not waves).
Would one claim super-resolution when refining the relativ
Begin forwarded message:
> From: "Charles W. Carter, Jr"
> Date: January 6, 2011 10:51:20 AM GMT+01:00
> To: Gerard Bricogne
> Subject: Re: [ccp4bb] FW: [ccp4bb] Resolution and distance accuracies
>
> I echo Gérard's thought.
>
> Pascal Retailleau did a relevant experiment published in Acta
Dear Axel,
On Sun, Dec 26, 2010 at 01:15:44PM -0800, Axel Brunger wrote:
> We defined "super-resolution" in our DEN paper as
> achieving coordinate accuracy better than the resolution
> limit d_min of the diffraction data. We proposed this
> definition in analogy to its use wide-spread use in
Dear All,
I would like to bring to your attention the call of
DK MOLECULAR ENZYMOLOGY,
AN INTERDISCIPLINARY BIOMOLECULAR PhD PROGRAM
at University of Graz and Graz University of Technology (Austria)
for 21 positions for graduate students.
The aim of the international Ph.D. program in Molecula
Dear All,
For those of you hoping to view the CCP4 Study Weekend online, we're
experiencing some technical difficulties with the webcast equipment.
Unfortunately the live stream won't be available for the first part of the
meeting. We're hoping to fix it as soon as possible and we'll send an up
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