Additives screening.
YB
2011-02-21
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发件人： Bosch, Juergen
发送时间： 2011-02-21 03:46:12
收件人： CCP4BB
抄送：
主题： Re: [ccp4bb] optimization of dumbbell like crystal
Seeding ?
changing from sitting to hanging drop or under oil ?
play with temperature ?
Jürgen
On Feb 20, 2011, at
Dear CCP4ers,
on behalf of Patrick Cramer, I send a Postdoc position offer, given
below. Please, do not respond to me, but to the e-mail addresses given
in the job description.
Best regards,
Dirk Kostrewa.
***
Applications are invited
In your original mail (2011-02-18; 18:45 GMT), you wrote: Possibly
deamidation of the protein, in particluar one or more lysines
Hence, my comment was based on your own writing, that is deamiDation.
Wishing you a better week.
Nadir
Pr. Nadir T. Mrabet
Structural Molecular Biochemistry
Dear Harvey,
Microseeding and adding 1-5% glycerol to the drops helped me when I
obtained crystals like these. Good Luck!
Bryan
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
Harvey Rodriguez
Sent: Sunday, February 20, 2011 9:29 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject:
Dear Harvey,
I had very good results with low melt agarose and the detergent screen
(e.g. Hampton Research). In both cases, the tendency for cluster formation
as well as the diffraction power could be improved.
Anja
Dear CCP4BBers,
Recently I got a crystal which appeared in the condition
Correct, the DS Visualizer is not available for Mac OS at this point, however
various emulators such as Parallels or VMWare have been used successfully.
Without going into too much detail, there is a new version (3.0) of the
visualizer which extends even further the capabilities of our previous
Dear all,
I got a lot of salt crystals in reservior solution (well solution), which
contains 0.1 M phosphate/citrate ph 4.2, PEG200 47%, EDTA-2Na 0-22mM.
Reservior solution appears crystals from 12mM EDTA. Could someone help me to
explain why?
Thank you very much!
Yibin
The pH is too low and the EDTA is insoluble. You need to be above pH ~8.0.
Your best bet is to try another chelator.
James
On Feb 21, 2011, at 2:22 PM, Yibin Lin wrote:
Dear all,
I got a lot of salt crystals in reservior solution (well solution), which
contains 0.1 M phosphate/citrate ph
