[ccp4bb] off-topic replay: glycerol in protein stock solutions

2011-03-05 Thread Alexandra Deaconescu
Hi Tom: Glycerol is fine in crystallization trials. As you say it may increase your solubility, in your case that appears to be a good thing, for other proteins, it makes them too soluble and then one may have to increase the concentration to something more than the typical values. Glycero

[ccp4bb] Philosophy and Re: [ccp4bb] I/sigmaI of >3.0 rule

2011-03-05 Thread Jrh
Dear Colleagues, Agreed! There is a wider point though which is that the 3D structure and data can form a potential for further analysis and thus the data and the structure can ideally be more than the current paper's contents. Obviously artificially high cut offs are both unfortunate for the