[ccp4bb] Structural Biology job vacancy

Structural Biologist – Crysalin Limited, Upper Heyford, Oxfordshire Crysalin Limited is a venture capital backed structural biology company recently spun out of the University of Oxford’s Biophysics Laboratory. It is based upon the pioneering work of Professor Martin Noble and Dr. John

[ccp4bb] about partial plroteolysis

hello CCp4ers: Sorry about the off-topic question. My protein is a kinase which the predicted kinase domain is from 32aa to 319aa. But the protein sequence after partial proteolysis is from 52aa. what should I do? Are there any reasonable expanation? Thanks in advance! Xinghua Qin -- Xinghua

Re: [ccp4bb] off topic: problematic protein

I would think thing here is that this protein actually associates to those lipid nanodiscs...(around the disc) and Na cholate CMC is around 10 mM. so, yes you can solubilise proteins that bind lipids, the question is does this protein bind lipids or not? or is it just scrambled or

[ccp4bb] Fwd: IUCr 2011 - AUGUST 22 - 30

Dear allI've been asked to distribute this in the best way I can - I can thinkof no better way than to distribute it to ccp4bb...Begin forwarded message:From: "IUCR" iucr2...@pacifico-meetings.comDate: 20 April 2011 09:27:45 GMT+01:00To: "'IUCR'" iucr2...@pacifico-meetings.comSubject: IUCr 2011 -

Re: [ccp4bb] about partial plroteolysis

On Wed, 2011-04-20 at 14:27 +0800, xinghua qin wrote: But the protein sequence after partial proteolysis is from 52aa. what should I do? Are there any reasonable expanation? The protease cut your protein at position 52 sounds reasonable enough to me -- Hurry up before we all come back to our

[ccp4bb] insert a cleavage site between scondary structures

Dear ALL; One of our proteins will gain activities after cleavage between secondary structures(probably at three sites). In the intact structure, the N-terminal and C-terminal domains beside this cleavage site have extensive contacts. So far we are stilling looking for the enzymes that

[ccp4bb] PDBe annotates its 10,000th structure

In June 1999, staff of the Macromolecular Structure Database (MSD; nowadays known as the Protein Data Bank in Europe, http://pdbe.org/) at the European Bioinformatics Institute (EMBL-EBI; http://www.ebi.ac.uk/) began annotating PDB entries. Now, eleven years later, PDBe has annotated its

[ccp4bb] 250 years of Bayes' Theorem: Link

This link should be helpful to many folks here: http://blog.revolutionanalytics.com/2011/04/250-years-of-bayes-theorem.html

[ccp4bb] Rcullis and phasing power

sirs: I am running BP3 in crank on SeMet data SAD and getting a solution, but we need a number like Rcullis which is nowhere to be found, and comes from Mlphare. What is best to report? thanks kas -- Kenneth A. Satyshur, M.S.,Ph.D. Associate Scientist University of Wisconsin Madison,