Re: [ccp4bb] No Observed SO4 in AmSO4 Crystallizations

2011-06-03 Thread Katherine Sippel
Hey Jacob, If you are looking for a specific example there are numerous carbonic anhydrase structures that were crystallized in ~2.8M ammonium sulfate that have no sulfate in the structure. Usually they have a ligand in the active site which displaces the only ordered sulfate molecule. I have foun

[ccp4bb] No Observed SO4 in AmSO4 Crystallizations

2011-06-03 Thread Jacob Keller
Dear Crystallographers, is it possible not to observe density for any sulfate ions in crystallizations done with molar-range sulfate concentrations? Beguiles the mind, but I seem to be looking at such a structure... Jacob -- *** Jacob Pearson Keller North

[ccp4bb] self rotation function

2011-06-03 Thread Careina Edgooms
Dear ccp4 members I have question about how to interpret polarrfn log. I wish to know if my crystal display NCS. I am not sure how to interpret the file. I see it have two peak, one is origin and the other is not that high to me. I have attach copy of the file. If someone could assist me to und

Re: [ccp4bb] larger molecular weight shown by analytic ultracentrifugation

2011-06-03 Thread Lionel
Dear Jerry, Just a few comments on your questions. "We tried both SV and SE. SV showed an increasing Sw with decreasing concentrations (Sw varied from 6.9, 7.0, 7.2, 7.4 to 7.522). IN addition, the SV experiments showed a single boundary. However, the boundary was a little broader, between 6