Hi all,
I have been trying to purify cytosolic fraction of membrane protein whose
domain boundries are unknown.
hence I have made a series of deletion constructs. The expression and
purification is not a problem.
I get good yields of the proteins. But on a gelfiltration column, they run
in the
I also observed the tcltk path problem when installing ccp4-6.2.0 on EL5
(CentOS 5 in this case). It happens if root is using tcsh instead of bash.
Notably, it must be a new bug introduced with the *revised* 6.2.0 packages. The
initial version (the one with the file permission problem) generated
Quoting you: I type ccp4 and I get no error, what happens if you type
ccp4i (followed by carriage return) ?
Yuri wrote:
I downloaded the package for RHEL5. My default shell is sh. But I can
change environments. I have progrmas that I must run in tcsh.
I tried sourcing sh and csh setups.
Dear All,
In crystal examination stereo zoom microscopes, how important is
it if one chooses a Achromatic objective lens compared to Apochromatic
lens.
Achromatic can give higher working distance ~ 70 mm or more as
compared to Apochromatic, which may be important for picking
On 07/30/11 04:05, Weiergräber, Oliver H. wrote:
I also observed the tcltk path problem...
I am not sure that it is the tcltk path he's talking about, it could be
the execution path. I think a look at the ccp4.setup file is needed to
figure out what went wrong.
