Dear CCP4Pers,
also j5 might be a good choice. I am not personally familiar with the
software, so I would happy to hear any comments. It also seems to cope with
modern cloning techniques like SLIC.
clicking away at http://j5.jbei.org/index.php/Main_Page
Regards,
Juha
On 27 September 2011 18:42,
On 09/29/2011 09:46 AM, William G. Scott wrote:
On Sep 28, 2011, at 5:26 PM, Jacqueline Vitali wrote:
Dear colleagues,
I need some advice for a new computer.
(1) I have the option of an HP Z210 8 GB with a low end Quadro Nvidia 400 512
MB.
--How does Coot run with this card?
OK
--I am
I use home built LINUX Intel boxes with 2Gb memory and low end Nvidia cards
(GT 8000 series to GT 200 series) and they are fine with Coot, Pymol, CCP4i.
I can even run CrysalisPro (Windows) in WINE. More memory is cheap to add.
If you use a Ubuntu LTS release, you get 3 yr of updates. Proprietary N
On Sep 28, 2011, at 5:26 PM, Jacqueline Vitali wrote:
> Dear colleagues,
>
> I need some advice for a new computer.
>
> (1) I have the option of an HP Z210 8 GB with a low end Quadro Nvidia 400
> 512 MB.
>
> --How does Coot run with this card?
>
OK
> --I am happy with any Linux. However,
On Wed, Sep 28, 2011 at 5:26 PM, Jacqueline Vitali
wrote:
> (2) Second option is an IMAC 4 GB 2.5 GHz with AMD Radeon HD 6750M 512 MB
> GDDRS.
>
> --How does Coot work with this graphics card?
>
I don't have exactly this, but something very similar (6770M), and it works
very well for me so far, b
Dear colleagues,
I need some advice for a new computer.
(1) I have the option of an HP Z210 8 GB with a low end Quadro Nvidia 400
512 MB.
--How does Coot run with this card?
--I am happy with any Linux. However, the system needs updates for security
purposes (the University requires it). Do
Structural studies on glutamate receptor ion channels:
A postdoctoral position in X-ray crystallography is available
immediately in the group of Dr. Mark L. Mayer, in the Laboratory of
Cellular and Molecular Neurophysiology at the NIH in Bethesda MD,
USA. This opening is part of an established
Hello CCP4,
Formulatrix has two job openings in Europe. A support engineer and a sales
engineer. Candidates may be located anywhere in Europe to be considered for
these positions. For more detailed information, including how to apply,
click here http://www.formulatrix.com/careers/careers-eu.htm
Susan and everyone,
I should apologise for any confusion that I may have caused.
Rajiv actually asked his question a year ago, and I accidentally replied to
it a year too late!
It's an interesting question though
Patrick
On Wed, Sep 28, 2011 at 5:03 PM, Linda Schuldt wrote:
> **
>
> Dear Raj
This paper on thermofluor is a good reference and if you have access to a
real time PCR machine, different buffer systems, like the PACT screen can be
evaluated within an hour to find out the buffer in which your protein is
most stable.
It gives the Tm of your protein and if you have a high fluores
Dear Raji,
what exactly do you mean when you say the melting temperature is 45deg.
Did you only test one buffer, or did you test many buffers and 45deg is
the most stable one? If you have only tested one buffer you should run a
screen testing different buffer systems (pH) and e.g. NaCl concentrati
On Sep 28, 2011, at 7:15 AM, Artem Evdokimov wrote:
> Are you indeed referring to the oil-filled high voltage transformer core in
> the older model generators?
Yes.
> (Newer ones tend to use solid state voltage multipliers).
That sounds much more sane.
> I recall that there were issues with th
We have proteins that melt at >60˚C but they don't crystallize. According to
your 45 degree rule we should have crystals, what are we doing wrong ?
Jürgen
On Sep 28, 2011, at 10:05 AM, Artem Evdokimov wrote:
For what it's worth, we've been using thermofluor to compare the 'apparent'
melting po
Are you indeed referring to the oil-filled high voltage transformer core in
the older model generators? (Newer ones tend to use solid state voltage
multipliers). I recall that there were issues with those things that were
eventually traced to elevated humidity. Now, that was humidity around 80%
whi
For what it's worth, we've been using thermofluor to compare the 'apparent'
melting points of enzymes with their thermal stability measured as
inhibition of their respective reactions by elevated temperature. The data
so far make sense - the differences in apparent enzyme Tm (using the same
conditi
We seek to recruit an outstanding postdoctoral scientist with strong
interest in Structural Biology/Structural Enzymology.
The main goal of this project is to illuminate the chemistry of
cobalamin biosynthesis in molecular and mechanistic detail exploiting
our recent discovery that several
I actually think you *can *make comparisons between different proteins. We
heard a very nice talk by Jose Marquez about exactly this at the RAMC
meeting recently.
Basically, 45C seemed to be the dividing line. If your protein melts below
this it's a bad sign for crystallization and may point to
We are looking for an outstanding PhD student to apply Terahertz
Spectroscopy to the study of protein librations and folding.
The studentship is funded at the standard research council rate for 3
years. A first class or upper second class degree (or equivalent) is
essential. For further det
You might like to look at this..
It tries to explain likely twinning possibilities in P21.
If you get C and P21, then probably a~=c - then Beta can have any
value.
C222 axes are then always possible with a* +c* , a*-c*, b* all
having angles ~ 90
Without twinning you wont get 222 sy
You wrote:
Original Message
Subject: P21/n spacegroup
Date: Tue, 27 Sep 2011 14:38:28 +0100
From: Franck
Dear all,
i'm struggling trying to integrate x-ray data in spacegroup P 1 21/n 1
(racemic mixture of peptides) with XDS or Imosflm. Can someone tell me
the best way to do
Hello folks,
A couple of years ago, we had a related discussion which I cut & paste
below. I still use Vector NTI which is expensive and bloated, but works
well. I've also had good experiences with Gentle which is free and open
source.
Darren
Thanks for the 38 replies, both on and off the bboard.
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