Re: [ccp4bb] detect dsDNA

Also this method might be useful if crystals tolerate the treatment. Regards, Juha Fluorescence detection of nucleic acids and proteins in multi-component crystalsActa Crystallographica Section D Volume 62, Issue 2, February

Re: [ccp4bb] detect dsDNA

If your DNA is small enough, you can run a control dsDNA lane on the gel. dsDNA will show up on silver stain but the oligomer has to be small enough to enter the gel. Kay On Sep 30, 2011, at 10:37 PM, zq deng wrote: > Hi all, > . > recently,I got a crystal of protein-DNA crystal.i used silve

Re: [ccp4bb] detect dsDNA

Some things that are a good sign for having DNA as well as protein in your crystal: 1) You get different (or no) crystals if you add or substract a base or two from the DNA ends. Of course, we did get fooled by this logic once when one particular oligo seemed to be contaminated with an easily

Re: [ccp4bb] detect dsDNA

I actually looked at an EtBr MSDS a while ago, and was shocked at how benign it was. I also heard from someone that they used to feed it to Argentinian cows routinely a few years back... JPK On Sat, Oct 1, 2011 at 1:56 AM, James Stroud wrote: > If you can reproduce the crystals and have the mate

[ccp4bb] Refinement with twinned data question

Dear all, I have some questions about the correct protocol for refinement of twinned data (0.46) 1- How do I ensure that my Rfree set is generated properly? 2-I noticed that if I enable intensity based twin refinement, refmac estimates the correct twin fraction law -h,-k,h+l. But for subseque