SELECTION OF POSTDOCTORAL FELLOW BY THE UNIVERSITY OF PATRAS
REQUEST FOR EXPRESSIONS OF INTEREST
[Greece]
[‘‘Ab initio’’ structure solution from electron diffraction data
obtained by a combination of automated diffraction tomography and
precession technique]
A post-doctoral position with a
Hi,
My DNA chain has a internal modification of 5-Bromo-2′-deoxyuridine (5BrdU).
I had a problem when I tried to model 5BrdU into my DNA chain by coot. I
will appreciate it very much if someone can solve it for me.
What I did...
1. Generate the cif dictionary and export the pdb for 5BrdU in
Hi Sabine,
Thanks for your suggestions.
I checked the cif file of BrdU.
the hydrogen of the 3'O of the BrdU is defined as HO3' in BrdU cif file, in
stead of 3'OH. The standard dNTP cifs define it as HO3' too.
In PDB, there is no atoms for hydrogen, as I excluded them.
The BrdU is defined as UBP
As Sabine said, you need to make sure that the monomer is defined as DNA
type. The best way to figure out how to do this is to look at the
standard monomer library for nucleotide, e.g.
$CCP4_LIB/data/monomers/d/DA.cif.
On Sun, 2011-10-23 at 18:44 -0400, zhang yu wrote:
Hi Sabine,
Thanks for
Announcement of the death of Herb Hauptman, co-winner of the 1985 Nobel Prize
in Chemistry, made by Ed Lattman and Walt Pangborn, Hauptman-Woodward
Institute, this evening:
It is with deep and profound sadness that we write to let you know that Dr.
Hauptman passed away Sunday afternoon. His
All
I have an O-style operator but when I put in the following under dmmulti, dm
refuses to continue to read the rest of my instructions...?
AVER -
REFI
omat
1.0 0.0 0.0
0.0 0.62 -0.008727
0.0 0.008727 0.62
-0.215399 -0.909085 -0.017797
XTAL 3
(it never reads XTAL 3).
Is
Hi,
Sorry for the extremely off topic question.
I was wondering if anyone had any opinions on which vector system is best for
ectopic expression of a protein of interest in human cells (quiescent or
dividing in tissue culture)? This would be for studies of the effect of the
protein on the
