Hi there
one thing I did to get more space for needles in a drop was to make
'book end' coverslips. Your take a plastic covership and with a razor blade you
cut obliquely into the plastic to make a 'flap' of the plastic. Then you lever
it up to vertical to give a projecting vertical s
On 11/15/2011 02:07 AM, Rojan Shrestha wrote:
Dear all,
I am trying to calculate the correlation between two 2Fo-fc maps but I was
getting error. The error is:
CCP4 library signal library_file:Cannot open file (Error)
This error was appeared after reading the given map files and trying t
Dear all,
mtzdump shows my space group as 'C 2 2 21' (number 20)
pointless gives error "Space group is not in library". part of the log file is
following.
I appreciate all the help.
Thanks
Raj
**
*
Dear CCP4 developers,
Forgive the posting here, but I can't find the correct contact details for
Graeme Winter…
I have just downloaded the latest version of XIA2 (0.3.3.3 build 3479) and
tried to integrate a dataset. The program fails with the following error…
>>>
Build: 3479
XIA2 0.3.3.3
Com
Thank you guys. I basically tried almost everything that I can find in the
hampton catalogue and in this bulletin, seeding, hanging, sitting,
sandwitch drops (which made things worse), temperature, gel, oil batch, and
additive screens. Manipulating the crystal with fiber increases the chance
to mak
Electron Microscopists: Center Director and Assistant Director
Indiana University, Bloomington
The Departments of Chemistry, Biology and Molecular and Cellular
Biochemistry at Indiana University, Bloomington seek outstanding
candidates for leadership positions in the newly constituted Electron
Hi all,
I came across this YouTube clip the other day that is well worth 3 1/2 minutes
of your time:
http://www.youtube.com/watch?v=Dhs1YO5nqXA
It was made by Tommy Christensen and shows how he built a model of DNA out of
LEGO bricks (of PDB entry 2DAU, to be precise) to celebrate th
Attached is a picture of making the bookend setup - step b. shows the razor
levering up the flap from the plastic coverslip - be careful not to cut right
through (I have done that and the drop dries extra quick in such a case) also
be careful to apply the protein/well droplet as shown with to t
Sent using BlackBerry® from Orange
-Original Message-
From: Nian Huang
Sender: CCP4 bulletin board
Date: Tue, 15 Nov 2011 12:44:28
To:
Reply-To: Nian Huang
Subject: Re: [ccp4bb] [off topic] Control of crystals' direction and position
in the drop.
Thank you guys. I basically tried a
Hi, there,
Maybe someone asked this question before, but I couldn't find it in the
archive.
We use the native data to do molecular replacement before, but only part of
the model fit the density. After collect a new set of selenoMet data, we try
to use it to solve the phase, it solve some of
Sharp will do that. Or you can simply handpick them when looking at your
anomalous map using the MR phases. With those sites you can use a variety of
programs to refine the SeMet sites and hopefully get better phases.
Are you sure your MR model is correctly placed ?
Have you tried conventional
How about Phaser with partial MR structure?
JPK
On Tue, Nov 15, 2011 at 3:56 PM, Feng Guo wrote:
> Hi, there,
>
> Maybe someone asked this question before, but I couldn't find it in the
> archive.
>
> We use the native data to do molecular replacement before, but only part of
> the model fit
Phenix does this
(http://www.phenix-online.org/documentation/autosol.htm#anch125)
Phaser-EP
(http://www.phaser.cimr.cam.ac.uk/index.php/Experimental_Phasing#Combined_MR_and_SAD_Phasing)
and SHELX using an experimental version you have to get from George
Sheldrick.
Jon
--
Jonathan P. Schue
I forgot to mention... there are two ways of calculating the phases in
Phenix, try both ways because the maps can look very different.
Jon
--
Jonathan P. Schuermann, Ph. D.
Beamline Scientist
NE-CAT, Building 436E
Advanced Photon Source (APS)
Argonne National Laboratory
9700 South Cass Avenue
A
Hi All,
I have an RNA-binding protein that I can purify out of bacteria in PBS
(Phosphate buffered saline;137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 2 mM
KH2PO4), but which is insoluble in Tris/NaCl-based buffers. My guess would
be that the inorganic phosphates (by mimicking RNA) are binding the prote
Hi,
This hasn't been mentioned by the people who have answered so far so here we
go: your molecular replacement solution and your SeMet solution to the phase
problem
are not necessarily using the same origin. There is a ccp4 program (is it
phistats ? and there may be other programs around) tha
Dear Tony,
Sorry you found it hard to track me down - I should add a note on the
end of the log file as to how to report errors :o)
In order to work out what happened please could you send me (off list)
the xia2-debug.txt and xia2.error files?
Thanks & best wishes,
Graeme
On 15 November 2011 1
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