Re: [ccp4bb] Angle between monomer of a dimer

2012-08-24 Thread Phil Evans
the angle of rotation is kappa = 160.045deg in this case On 24 Aug 2012, at 04:33, Appu kumar wrote: Sir one more help plz i have the following matrix which one shall i use to calculate the angle. INFO:: coordinates transformed by orthonal matrix: | -0.6927, -0.7191,0.0564| |

Re: [ccp4bb] d*trek

2012-08-24 Thread Harry Powell
Hi It should do. Jim will know for sure. On 23 Aug 2012, at 23:08, jlliu liu wrote: My real questions is: Does d*trek recognize the img file format collected at ALS or APS and process the data? Thanks! On Thu, Aug 23, 2012 at 6:00 PM, jlliu liu jlliu20022...@gmail.com wrote:

Re: [ccp4bb] Angle between monomer of a dimer

2012-08-24 Thread Paul Emsley
On 23/08/12 20:08, Nikolina Sekulic wrote: Dear Appu, I had similar issue once. You can use Coot to overlay two structures and get the rotation matrix a1 a2 a3 b1 b2 b3 c1 c2 c3 from here you can calculate cosine of an angle using formula (a1+b2+c3-1)/2 You can if you like, but Coot does

Re: [ccp4bb] Angle between monomer of a dimer

2012-08-24 Thread eugene . krissinel
I believe $CCP4/bin/superpose does the same Eugene On 24 Aug 2012, at 11:44, Paul Emsley wrote: On 23/08/12 20:08, Nikolina Sekulic wrote: Dear Appu, I had similar issue once. You can use Coot to overlay two structures and get the rotation matrix a1 a2 a3 b1 b2 b3 c1 c2 c3

Re: [ccp4bb] d*trek

2012-08-24 Thread Jim Pflugrath
d*TREK can process single crystal diffraction images from all the common detectors including not only Rigaku detectors, but many different detectors found at synchrotron beamlines around the world such as the APS, ALS, NSLS, CHESS, SLS, ESRF, Diamond, Soleil, PhotonFactory, SPring8, CAMD,

[ccp4bb] structural homology

2012-08-24 Thread amro selem
Dear all, i want to do structural homology but i am still beginner , could some one help me to do it.is there are specific  programs to do that or should i do it manually ? thank you Amr

Re: [ccp4bb] structural homology

2012-08-24 Thread Bosch, Juergen
I-tasser or Robetta , modeler and many more Sent from my iPad On Aug 24, 2012, at 22:23, amro selem amro_selem2...@yahoo.commailto:amro_selem2...@yahoo.com wrote: Dear all, i want to do structural homology but i am still beginner , could some one help me to do it.ishttp://it.is there are

Re: [ccp4bb] structural homology

2012-08-24 Thread Das, Debanu
Adding to the popular suggestions.. Check out the PSI Protein Model Portal (http://www.proteinmodelportal.org/), which allows submission to several servers including I-Tasser and Modeller as well as some informative tutorials on modeling workflow, etc. Also check out some other standalone

[ccp4bb] Improving microcrystals

2012-08-24 Thread Samuel Johnson
Hi everyone,   I have been working on a protein for the past year. After a number of trials at crystallizing the protein i have identified conditions for getting spherulites/micro-crystaline material under micro batch method. I have confirmed that the crystalline material is

[ccp4bb] Off-topic: Duet vectors with C-terminal GST tag

2012-08-24 Thread Lye, Ming
Dear CCP4bb, We would like to co-express proteins under Se-Met conditions for de-novo phasing of a complex. One of the proteins expresses much better with a GST-tag compared with a his-tag. As its binding site is at its N-terminus, we are hoping to co-express it as a C-terminal GST tag