Yuri
Whatever happens, keep the remains of the crystal to make a seed-stock
after data collection.
You can also try making a seed stock from the rest of the drop, even if you
can't see crystals, and even if it has dried up.
Use part of your seed-stock to seed into one or more *random screens*
Y
If that solution applies to the original model you used (and not to re-solving
the structure with the molecular replacement solution before or after
refinement), then your tetramer model is just being rotated by 90 degrees
around the 4-fold and placed on a different origin, i.e. the solution is
On Fri, 30 Nov 2012 17:27:41 -0500, Sarathy Karunan Partha
wrote:
>Dear all,
>
>Here is the XSCALE.LP output after scaling for the izit dye stained
>crystak. Sorry for attaching the .INP file.
>
>Sarathy
>
>On Fri, Nov 30, 2012 at 4:19 PM, Sarathy Karunan Partha <
>sarathyus...@gmail.com> wrote:
Dear Sarathy,
I was going to reply along the same lines as Kay Diederichs just
did. At the Cr K-alpha wavelength, you would have strong absorption
effects which, given the low symmetry of your crystal, would be very
hard to get rid of. We have seen numerous cases where a high anomalous
correl
These look like spherulites. They are often optimizable into crystals by
varying the pH, precipitant/protein concentration,
additives,crystallization temperature, etc.
___
Roger S. Rowlett
Gordon & Dorothy Kline Professor
Department of Chemistry
Colgate Unive
Definitely magnesium salt. I've seen it many times. But try to repeat it
anyway just to be safe.
Nian
On Sat, Dec 1, 2012 at 8:24 PM, Yibin Lin wrote:
> Dear Folks,
>
> I got some crystals at the conditions of 0.1M Lithium sulfate or 0.1 M
> MgCl2, 0.1M Sodium citrate 3.5 or Sodium acetic ph5.5
