Dear Gerard,
Many thanks for these useful clarifications.I see your points clearly.
Just to mention that one remark in James's posting regarding photon counting
versus read noise caught my attention. I will follow up on this ASAP , which
like fine phi slicing gets to the heart of the
Research Assistant – Membrane Protein Production/Crystallization
Membrane Structural and Functional Biology – Caffrey Lab
Trinity College Dublin, Ireland
Post Summary
The Membrane Structural and Functional Biology group seeks to solve the crystal
structure of medically important membrane
Dear all,
I have a crystallization-related question. I am going to co-crystallize
protein with RNA. I ordered a short (10 mer and 14 mer) RNA from Dharmacon
but did not choose the purification option while ordering them due to the
budget issues. How critical is to HPLC purify them before setting
Dear Alex,
In my experience it is essential to HPLC purify short-RNAs for crystallisations
as the impurities will hinder your crystallisation attempts.
Best wishes,
Salima
Dr. Salima Nurmohamed
Division of Structural Biology,
Wellcome Trust Centre for Human Genetics,
University of Oxford,
On May 20, 2013, at 7:20 AM, A K alek6...@gmail.com wrote:
Dear all,
I have a crystallization-related question. I am going to co-crystallize
protein with RNA. I ordered a short (10 mer and 14 mer) RNA from Dharmacon
but did not choose the purification option while ordering them due to the
Hi Alex,
If you do not have access to HPLC equipment, another alternative is
gel-purification using a PAGE setup under denaturing (urea) conditions.
This has the advantage of being fairly simple and effective for a range of
transcripts, but you will need a fairly large gel tank system to get good
