Dear all,
I was wondering if it sounds logical to use the crystals from a possible
structural homolog as seeds to induce nucleation ? (in terms of overall
sequence, the proteins are considerably different but based on sequence
alignment and structures from other related proteins, it is highly like
I do not have experience with this but it is worth trying.
On Fri, Dec 27, 2013 at 2:29 PM, Mahesh Lingaraju wrote:
> Dear all,
>
> I was wondering if it sounds logical to use the crystals from a possible
> structural homolog as seeds to induce nucleation ? (in terms of overall
> sequence, the
the differences are likely to be on the protein surface, and these would make
the crystal contacts, i.e. it would be unlikely the protein could crystallise
in the same crystal habit.
Never say never in crystallisation (i.e. try anything), but I would go for
other things first like additives, dif
I know of at least one published example where seeding has been used to
crystallise a number of homologues:
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2243101/
(Sanchez-Weatherby 2006, Acta Cryst F 62:518)
There are probably others out there...
Certainly worth giving it a go.
Best wishes,
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