Thank you all for your suggestions. I try various methods suggested and
hope they work.
Regards
Venkat
On Wed, Jan 22, 2014 at 11:44 PM, Roger Rowlett rrowl...@colgate.eduwrote:
Thrombin cleavage of His-tags seems to work pretty well in our hands,
which includes mostly undergraduates for
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Dear all,
could anyone suggest a way to get the average B-factor from a PDB-file
of those atoms a specific ion binds to (e.g. as judged by header LINK
records or a distance interval)?
I would like to get this number for all K-ions from a set of
Hi
Does any one know how to fix this ERROR in xds.
!!! ERROR !!! CANNOT READ X,Y-CORRECTIONS.cbf
regards
Saleem
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Dear Saleem,
you need to run the XYCORR step at least once (by placing this word on
the JOB card in XDS.INP).
There is very good documentation about XDS available at Wolfgang
Kabsch's web site http://xds.mpimf-heidelberg.mpg.de/ (and of course
the
Gabriel,
Could this be just different but equivalent way of defining the asu? Ignoring
one of the two tetramers and just focusing on the one tetramer that looks
different in your case, the following picture assumes objects ABCD form a
tetramer and repeat themselves in P1. You can have one
If Paratone oil is too viscous, I recommend perfluoropolyether (PFPE). A lot
of small molecule groups use this because it reduces damage to the crystals.
Kris F. Tesh, Ph. D.
Department of Biology and Biochemistry
University of Houston
From: Kelly Daughtry
Dear Tim,
Thanks for the help. It is working now.
regards
Saleem
Date: Thu, 23 Jan 2014 16:40:52 +0100
From: t...@shelx.uni-ac.gwdg.de
To: mysaleemr...@hotmail.com
Subject: Re: [ccp4bb] XDS Error message
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Dear Saleem,
I agree with you
Hi Tim,
a non-ccp4 solution (since you haven't gotten any suggestion yet)..
1) Get atom selection of atoms involved into ion coordination:
phenix.metal_coordination model.pdb
2) Using atom selection from above extract portion of PDB that contains
atoms in question:
phenix.pdb_atom_selection
