Dear
all,
this is a reminder that the deadline for application to the EMBO Practical
Course Protein expression,
purification and characterization/crystallization (PEPC9) is 18th of May. The
course will
be held at EMBL Hamburg from the 8th of
September until the 16th of September 2014.
This
is
Speed of a computer language can mean two things. Seconds from run command to
result depends on the quality of the language implementation. Months from
hiring to publication depends on the quality of the language design. Which of
these matters most depends on context.
Date:Thu, 8 May 2014
Dear Ccp4,
I wondered if anyone has experience with a Knauer Bioline FPLC system for
the purification of proteins. On paper, the Knauer system seems a good
alternative to the more commonly used Aekta and (NGC) Bio-rad system. As
there are not many Bioline Knauer systems around I would
Can it be parallelized? That is how you reduce run-time. One of the tests
matrix-matrix multiplication has been successfully speeded up by using GPUs.
CUDA is the language used for this, which is a derivative of C. To be fair you
only see the benefit for really large matrices, smaller ones
Le 09/05/2014 15:36, Adam Ralph a écrit :
Can it be parallelized? That is how you reduce run-time. One of the
tests matrix-matrix multiplication has been successfully speeded up by
using GPUs. CUDA is the language used for this, which is a derivative
of C. To be fair you only see the benefit
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Hi Pascal,
I would think that a good programmer can often optimise better for a
specific problem than a general purpose library would. For example I
implemented a peak search for diffraction images which is about an
order of magnitude faster than
Dear Herb,
These things happen.
I have been interpreting the CBF coordinate system as being a
generalised form of the d*TREK one. I read the definition on page 26 of
the document that I have found here:
http://www.rigaku.com/downloads/software/free/dTREK%20Image%20Format%
20v1.1.pdf
to
Dear Maria
have you collected the crystals before they dissolve and washed them, then
either dissolved them in SDS PAGE buffer and ran them on an SDS PAGE gel (for
say a western blot) or capillary mounted them, then shot them on the x-ray set
to determine if they are protein or small
Dear all,
Why does (if it is suppossed to do so) Superpose output results for a
subset of atoms only? See a summary of log file below (just the top
lines, data on atoms, and final data and message). In the example,
results for residues 69-76 are absent, other atoms are absent as well,
I should have said that this is the comparison of two crystaline
states of the same molecule, the sequences are identical.
Best
H
hbo...@pasteur.edu.uy ha escrito:
Dear all,
Why does (if it is suppossed to do so) Superpose output results for a
subset of atoms only? See a summary of log
Dear H,
you are referring to superpose, but your logfile lists the output from
LSQKAB, which are two different programs. According to the man-page of
superpose, it uses secondary structure elements for superposition, so
maybe the missing atoms are those not part of a helix and not part of a
Hi Maria,
Adenosine is quite stable but not very soluble in aqueous solution. A
labmate of mine would routinely observe adenosine precipitating in his
crystal trays, which later dissolved, presumably as his protein slowly took
up ligand.
To follow the previous comments, I'd make sure what you
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