Re: [ccp4bb] The discrepancy for determination of Ramachandran outliers by Coot and MolProbity

2015-02-19 Thread Gert Vriend
On 19-2-2015 1:38, Smith Lee wrote: Dear All, It often finds for the Ramachandran favored determined by Coot, MolProbity regards as Ramachandran outliers. There are earlier posts regards Coot and MolProbity has different database for the determination of the Ramachandran plots. Then will you

[ccp4bb] Thank you everyone! - Poor experimental phases for a largish structure...

2015-02-19 Thread William Chao
Dear all, Thank you so much for all the replies and suggestions in such a short period of time since I posted my questions! I am slightly overwhelmed by the number of things that I could try in the near/medium future! One thing that I am recommended a lot is TaBr. It seems that this compound

[ccp4bb] Density fit analysis in Coot, and FEM

2015-02-19 Thread Emilia C. Arturo (Emily)
Hello all. I'd like to understand what it is I'm looking at when I use Coot's density fit analysis tool. I recognize that there was a post related to this topic on the Coot bb a while ago --the discussion was on how to interpret the red-ness or green-ness of the density fit plot (

Re: [ccp4bb] Can nucleoprotein complex inhibit EtBr binding?

2015-02-19 Thread Phoebe A. Rice
Yes it can. Ethidium stains DNA by intercalating between base pairs, so if most of your DNA is tightly bound by protein the ethidium may not be able to wiggle in there. We've seen that happen with Mu transposase - DNA complexes. If that's the problem, soak your gel in SDS plus EtBr and you

[ccp4bb] Thymine methyl in coot

2015-02-19 Thread Phoebe A. Rice
When trying to adjust the chi angles of a dT in coot 0.8.1, the methyl and its H's (which I called C5M to make phenix happy) rotate with the sugar, producing a rather base bizarre geometry.