[ccp4bb] How can I refine B-factors only in CCP4 REFMAC?

Dear all, How can I refine B-factors only in CCP4 REFMAC? I do not want to refine the XYZ coordinations. Thanks Peng

Re: [ccp4bb] SeMet in S2 cell

Dear Scott and the board, Sorry for the email not intended for all. This is a really useful protocol for S2s; we so use slightly different ways of making protein in S2. My students will takes notice of this! Best, Engin On 12/23/16 5:56 AM, Engin Özkan wrote: Hi guys, Here is a protocol

Re: [ccp4bb] SeMet in S2 cell

Hi guys, Here is a protocol about use of S2s and SeMet labeling from the ccp4bb (the crystallographers' friendly bulletin board for knowledge exchange). I suggest you read the thread starting from the bottom to follow the logic. There will be useful information there. Best, Engin On 12/23

Re: [ccp4bb] New version of Adxv (1.9.11) available

A new version of Adxv (1.9.12) is available: http://www.scripps.edu/~arvai/adxv.html#Download The manual is here: http://www.scripps.edu/~arvai/adxv/AdxvUserManual.pdf The only difference between this and the previous version (1.9.11) is that CrystFEL geometry files are no longer supported. The

Re: [ccp4bb] SeMet in S2 cell

Nothing specific about S2 cells to contribute... However, S2s usually grow fine in other insect media, such as those intended for lepidopteran cell lines like the Sf9 and High Five. Protocols established for those cell lines, for example, check out those using the medium ESF-921, would probably

[ccp4bb] Why TLS refinement may go wrong and why it is so often the case (was: Re: [ccp4bb] Calculation of generalised R-factor?)

Dear Ethan, thanks for comments. I do remember we talked about it last year in Argonne at CCP4 school and we did not seem to agree indeed. Let me bring my arguments as you stated yours. Refinement is an optimization problem that involves model, data and fitting tools (such as target function and

[ccp4bb] Spring 2016 MicroED workshops at Janelia Research Campus

Due to high demand, two back-to-back MicroED workshops will be held at Janelia Research Campus (Ashburn, VA) in the spring. The workshops are hosted by Tamir Gonen and will have the same content. Accepted participants will be assigned by the organizers to one or the other (not both). May 21

Re: [ccp4bb] Shipping crystals for RT data collection

Hi All, Brings back a lot of memories about what can go wrong - some of them lessons hard learned transporting crystals in collaboration with Gloria! I’d echo Gloria’s advice and go further recommending the Hampton Research capillary cutter, Tygon tubing that fits the end of the capillary, a s

Re: [ccp4bb] Shipping crystals for RT data collection

I would recommend just mounting them in capillaries without loops, as this will travel better. Put slugs of mother liquor on either side, seal with wax and then coat wax and onto the end of capillary glass lightly with fingernail polish. We have had terrible luck with FedEx delivering damaged crys

[ccp4bb] Shipping crystals for RT data collection

Hi all, I'm looking for a way to ship crystals for room temperature data collection. I want to ship them already mounted on loops with capillaries, not in drops like the In situ trays from Mitegen. Any experiences on that? Thanks -- Ricardo Padua HHMI Postdoctoral fellow Kern Lab Brandeis Un

[ccp4bb] Scientist position with the Hauptman-Woodward High-Throughput Crystallization Center

Dear All, Please pass on the information about this position to all who may be interested. Best wishes for the season and a Happy New Year. Eddie The Hauptman-Woodward Medical Research Institute (hwi.buffalo.edu) operates the High-Throughput Crystallization Screening C

[ccp4bb] Job opportunity - Structural Biology GSK Vaccines

Dear ccp4 community, The Structural Biology Lab at GSK Vaccines, Rockville, MD, USA, is seeking a candidate for a Scientist/Senior Scientist position (Job ID: 148543, hiring managers: Enrico Malito, Matthew Bottomley). We offer outstanding opportunities and compensation to outstanding candidates.

[ccp4bb] Postdoctoral position at the University of Leeds

Somewhat off-topic, but nonetheless: There is a position at the University of Leeds for a Molecular Parasitologist, working on identifying new potential targets in Plasmodium falciparum by CRISPR/Cas, with the eventual aim of taking them forward for structural/drug design studies. The position