Dear ccp4ers,
I was wondering about most recent advances in production and
crystallization of complexes of multisubunit membrane proteins, for
instance succinate dehydrogenase, pyruvate dehydrogenase complex or even
photosystem complex. As far as I know, many large complexes of membrane
pr
Hi
I am looking for some literature or the structure of the metallopeptidases
where the substrate bound with the ezyme but with shifted P2-P1-P1'-P2'
cleavage site. May be some theoretical case study is there.
Best
Jiri
Dear all,
Please mind the approaching deadline (= 6th February 2017) for
conference registration at the 5th Banff Meeting on Structural Dynamics
https://banff2017.desy.de/
Best regards,
Irmtraud Kleine
Assistant to Professor Chapman
Deutsches Elektronen-Synchrotron DESY
Ein For
Speaking specifically for succinate dehydrogenase, there are a number of
assembly factors required for insertion of flavin, iron-sulfur clusters
(SDHAF1,2,3 . . .). Since E. coli or Pischia make their own SDH, there is
a possibility the endogenous assembly factors and co-factors would work
with t
Hi,
Sorry for another non crystallography question. Can someone suggest a fraction
collector to collect fractions from a CsCl/glycerol/sucrose/... gradient from
an ultracentrifugation run? Thanks.
Best wishes,
Reza
Reza Khayat, PhD
Assistant Professor
City College of New York
Department of Che
Hi Reza,
Are there certain requirements for the collection of the fractions you need
from the UC run or do you just want advice on any type of collector?
For just regular collectors, have you tried eBay? I have had success
purchasing used equipment from there. A quick search turned up an old
Ph
Reza,
Almost any fraction collector will do. If you have a pack rat colleague around
NY City, find an unused, but working Gilson fraction collector, with a rack
(which I surprising number of people use without knowing what the rack is for).
In our teaching lab we have about 7 of them we occas
I’m looking at my copy of Terrance Cooper’s ‘The Tools of Biochemistry’ (Wiley,
1977). It identifies two approaches to fractionating gradients. The first is
the one Michael describes (allowing the liquid to drip out of the bottom). The
other is to pierce the bottom of the tube, and then pump a v
