Re: [ccp4bb] Refmac: removing selected ligand hydrogens after making a link

After outputting the hydrogens, delete the ones you don't want and from then on do refinement with: make hout Y make hydr Y When you do that, refmac will keep the hydrogens you put in, and also put them in the output. -James Holton MAD Scientist On 3/29/2018 2:11 PM, Phil Jeffrey wrote: I

[ccp4bb] Postdoctoral position in cell division at the Wellcome Centre for Cell Biology, Edinburgh, UK

Dear All, A Wellcome Trust funded postdoctoral position is available in our group (http://jeyaprakash.bio.ed.ac.uk) at the Wellcome Centre for Cell Biology (www.wcb.ed.ac.uk), University of Edinburgh to structurally characterise protei

[ccp4bb] Refmac: removing selected ligand hydrogens after making a link

I've got a couple of instances where I have non-standard amino acids, nevertheless present in the monomer dictionary, that have additional non-peptide covalent linkages. I've figured out how to define these, but if I opt to output hydrogens as a diagnostic I see that Refmac doesn't delete the

Re: [ccp4bb] point group...321

Look at CCP4 v7.0 Program Documentation reindexing Yes PG 321 has two indexing options.. - *i.e.* reindex (h,k,l) to (k,h,-l) which is equivalent here to reindexing (h,k,l) to (-h,-k,l). On 29 March 2018 at 21:26, Gihan Ketawala wrote: > Hi, > Can the point group 321 show indexing am

Re: [ccp4bb] point group...321

Yes, several, but it's only important when merging multiple data sets. And it's not really ambiguities but alternatives. JPK + Jacob Pearson Keller Research Scientist / Looger Lab HHMI Janelia Research Campus 19700 Helix Dr, Ashburn, VA 20147 Desk:

[ccp4bb] point group...321

Hi, Can the point group 321 show indexing ambiguities? if not since I'm dealing with non-anomalous dataset can I use 3m1 instead? Thank you, Best, Gihan

[ccp4bb] RA position in London

Dear all, our lab is recruiting a research assistant to work on the COPII coat using biochemistry and structural cryo-EM. The purpose of this job is to provide support to the group and assist research in the lab, and we are looking for an enthusiastic person who has experience in protein bio

Re: [ccp4bb] A new capability on the STARANISO server: "PDBpeep"

Dear Jacob and Gerard, we performed a statistical analysis of anisotropy of the entire PDB, with a special focus on membrane proteins (Jacb, your other post). You can find more details on this article: https://www.nature.com/articles/s41598-017-17216-1 We also performed an analysis by space g

[ccp4bb] PhD studentship - Glasgow University/Astra Zeneca

Dear Colleagues, Applications are invited for a PhD studentship position funded by the BBSRC-CASE scheme in the laboratory of Dr Laura Spagnolo, University of Glasgow, in collaboration with Dr Taiana Maia de Oliveira, Astra Zeneca, Cambridge. The project involves the cryo-electron microscopy stu

[ccp4bb] Post-doc position at AstraZeneca

Dear all, We have an opening for a Post-Doc Fellow in CryoEM/Structural biology at AstraZeneca in Gothenburg. The aim of the project is to study multi-protein complexes of relevance for cellular signaling. The project will be a collaboration with Dr. Alexey Amunts at SciLifeLab, Stockholm Unive

Re: [ccp4bb] Are there reliable methods for Selenomet incorporation in Pichia ?

> > hi > you can find details in this manuscript on how to label recombinant proteins > produced by P. pastoris > Crystal structure of the effector AvrLm4-7 of Leptosphaeria maculans reveals > insights into its translocation into plant cells and recognition by > resistance proteins.