Hello,
I second Sudipta's point. I had a dataset at 3.3 angstrom resolution that was
initially indexed as P21 21 21, but did not produce a convincing MR solution.
The trick was performing the MR search for all possible spacegroups in the
pointgroup. The correct spacegroup was P2 21 21.
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Hello Zhou,
How sure are you about the space group of the solution? Any signs of
twinning or t-NCS?
Best,
Sudipta.
On Mon, Aug 20, 2018, 7:36 PM SUBSCRIBE CCP4BB Zhu Qiao <
jasonqia...@gmail.com> wrote:
> Hi All
>
> My protein is dimer both in protein buffer and crystallisation reservoir,
>
?Hello Zhu,
The implication being that one can have half of a dimer in the asymmetric unit
without any issues (biological or crystallographic). And having a R/Rfree after
a bit of refinement of the values you report for a 3 A data set is not that far
off from being appropriate.
Best of
75% solvent is not uncommon, and such crystals often only diffract to 3A.
Eleanor
On 20 August 2018 at 10:24, SUBSCRIBE CCP4BB Zhu Qiao wrote:
> Hi All
>
> My protein is dimer both in protein buffer and crystallisation reservoir,
> which is confirmed by calibration column Supderdex 200 10/300
Hi All
My protein is dimer both in protein buffer and crystallisation reservoir, which
is confirmed by calibration column Supderdex 200 10/300 increase.
The crystal can diffract to 3 and the space group was determined to be P212121.
The solvent content and Matthews coefficient shows
1 copy,
We focus mainly on non-H clashes.
Careful inspection of H-clashes can sometimes lead to an improvement of the
structure (better conformer). Very often we find the listed H-clashes
"unavoidable", i.e. under the given data and converged refinement we cannot
do anything anyway. So a very serious