On 04/11/2019 17:06, Pavel Mader wrote:
Hi Paul,
thank you for your answer. My problem is that I am building a cyclic peptide with non-standard amino acids,
the side chains of which are linked by click chemistry... I know how to make modified amino acids that will
make peptide bonds with
Hello Eleanor,
We tried to follow your suggestion.
The atoms to be linked were 1.46A apart in the input pdb model and we ran 10
cycles of refinement in Refmac with the "make link between" option chosen in
either "defined in the file or residues are close". After the refinement we
saw the
Hi all,
A reminder that there is only a week left to apply for a place at the RapiData
2020 @ SSRL workshop. Applications close the middle of this month, November 15
2019.
The workshop will be held from March 30 - April 4 2020 on the SLAC campus in
Menlo Park, California. The course will
What does refmac do if you feed it the coordinates with side chains close
enough to bond? In some cases it makes a full link dictionary with bonds
angles planes etc. But can coot read such a dictionary entry?
On Mon, 4 Nov 2019 at 17:06, Pavel Mader wrote:
> Hi Paul,
>
> thank you for your
Hi Paul,
thank you for your answer. My problem is that I am building a cyclic peptide
with non-standard amino acids, the side chains of which are linked by click
chemistry... I know how to make modified amino acids that will make peptide
bonds with their neighbors in the polypeptide chain, but I
Dear Michael
The Rmerge in the strong intensity bin of 0.079 is untypically high it seems to
me.
Were the diffraction images underexposed?
Best wishes
John
Emeritus Professor of Chemistry John R Helliwell DSc_Physics
> On 3 Nov 2019, at 23:19, Michael Jarva wrote:
>
>
> Hi CCP4BB,
>
>
Dear all,
indeed, crysalis can import multiple image formats. I was having trouble
with finding a dummy parameter file that would allow me to import my
dataset. Once this was done, a real parameter file was created with the
info from my dataset's image headers.
Thank you very much for your
Hi Everybody,
I am also interested in this, but the current version of CrystalisPro that we
have does not read HDF5 data files from EIGER detectors. Currently we convert
to CBF, but Crystalis still has issues… Does anybody have a sure-fire script to
convert HDF5 to Esperanto?
Cheers,
Bill
The current version of CrysalisPro will directly import several image
formats, or you can convert images to Esperanto format to import. The
latter is a bit clumsy but does work.
__
Roger Rowlett
On Mon, Nov 4, 2019, 9:54 AM Almudena Ponce Salvatierra <
maps.fa...@gmail.com>
Dear all,
does any of you have experience with using Crysalis Pro software from
Rigaku with data that were not collected on a Rigaku instrument?
Any help will be much appreciated.
All the best,
Almudena
To unsubscribe
Hi Jacob,
An additional technique to identify sodium is microPIXE, which can be used for
elemental mapping. I'm not sure if the sulfur signal would be strong enough to
be quantitative.
A ref: https://www.cell.com/structure/pdf/S0969-2126(00)88335-5.pdf
Hope that helps,
Sarah
Sarah EJ Bowman,
My first check would be to inspect the data processing plots -
Wilson Plot? Is it normal? predicted B factor of 8 - why?
Then the plot from refmac and v resolution?
Is there a scaling abnormality?
B of 8 is surprisingly low for that resolution but maybe the crystals just
did not have enough
Dear colleagues,
We are pleased to announce the opening of the first CCP4 Study Weekend
illustration competition, made possible by CCP4’s long standing
collaboration with IUCr Journals.
Getting your science out to the public requires more than good research.
You need to make it memorable! And
Senior Scientist Structural Biology (GPCR; cryo-EM; Xrays; SBDD; Biophysics)
(Senior) Research Associate Structural Biology (GPCR; cryo-EM, protein tool
generation, biophysical/biochemical screening)
We are looking for a highly motivated individuals to join our growing
structural biology team
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