[ccp4bb] OFFTOPIC question "Two plasmids in one host cell"

Hi All, I have two constructs having different ori, p15ori and M13 ori, different promoters araBAD promoter and LacI, and different antibiotic resistance chloramphenicol and Ampicillin respectively. I am managed to get the transformants and getting the expected result after blunt digestion with th

Re: [ccp4bb] CCP4BB Digest - 29 Dec 2020 to 30 Dec 2020 (#2020-345)

Hi Anamika, Just to be clear, the M13 ori is for production of ssDNA using a helper phage. Replication of that plasmid is more likely from another ColE1-derived origin elsewhere on the vector and also likely to give high copy numbers - which it seems it does. The p15 ori is indeed quite a low c