Thank you Prof Raaij.
I also have bad experience with sonication for one proteins. It denatures after
lysis with sonicator (1sec on/off pulse, 30% amp, 5 min). However, when we lyse
the cell with beads (vortexing with beads for 1 min, 5 times), we were able to
get good protein.
Best Regards
Exploring Self-Assembling DNA Crystal Scaffolds for the Precise Arrangement of
Biomaterials
Tara MacCulloch, Arizona State University
Center for Molecular Design and Biomimetics at the Biodesign Institute
Wednesday March 24; 1:30 pm
LINK:
Hi,
A postdoctoral position and a research staff position are available in the
Enemark lab for highly motivated applicants to study the structure and function
of helicase proteins and their mechanism of action on nucleic acids. For
representative publications, see:
M. Meagher*, L. B.
Sorry, I mistyped the old XDS site address. Correct is
http://xds.mpimf-heidelberg.mpg.de/ .
best,
Kay
On Tue, 23 Mar 2021 17:12:33 +, Kay Diederichs
wrote:
>Dear academic users of XDS,
>
>the latest version of XDS has its release notes now at https://xds.mr.mpg.de
>(the old
Dear academic users of XDS,
the latest version of XDS has its release notes now at https://xds.mr.mpg.de
(the old xds.mpimf-heidelberg.de can still be used but is redirected).
The download location can also be found there.
If your current (academic license) version of XDS is from last year,
Dear all,
Thank you for the kind offers of PEG, and for the helpful suggestions.
I'm hoping a return to Fluka-branded PEG will solve my issues, but if it
doesn't, I'll be sure to follow up on some of the other suggestions.
Thanks again,
Galen
From: CCP4
Hi Saurabh,
I have also encountered a similar problem. Sometimes, though your protein
looks pure in size exclusion chromatography, but actually is not very pure
and may contain traces of partially unfolded form or some transient
oligomeric forms. In such a case, your size exclusion profile would
During a prior period of great concern about variable peroxidation levels of
commercial PEGs (and of polyoxyethylene detergents), we measured/monitored
peroxide levels of solutions. The Pierce kit is now available from Fisher
just one hour left !!
https://twitter.com/jbiolchem/status/1374030429729271811?s=20
--
Dr. Javier M. González
Instituto de Bionanotecnología del NOA (INBIONATEC-CONICET)
Universidad Nacional de Santiago del Estero (UNSE)
RN9, Km 1125. Villa El Zanjón. (G4206XCP)
Santiago del Estero. Argentina
Dear All,
Sorry for the off topic.
May I ask your experience about bacterial cell lysis using glass beads beating?
How is the cell lysis compared with sonication and French press? Does the glass
beads affect the protein quality?
Thank you in advance.
Best Regards
Veerendra Kumar, PhD
Associate
Dear all,
We would like to share with you the press release of the recently EU
granted MOSBRI (Molecular-Scale Biophysics Research Infrastructure ).
MOSBRI will connect leading molecular biophysics facilities across 11
countries, including 13 academic centres of excellence and 2 industrial
can confirm jon´s comment. I find these in virtually every high-res structure.
some of them wobble a bit given the AA close by, such as Arg.
Dr. Matthias Barone
AG Kuehne, Rational Drug Design
Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP)
Robert-Rössle-Strasse 10
13125 Berlin
This doesn’t surprise me in the slightest, given what how the two maps are
calculated.
The polder map is essentially the same as your "simple omit map” without the
bulk solvent contribution that is present in the omit-refine map. Since this is
an approximately constant contribution, if you
Hi Galen,
Is it possibly the age of the original material, rather than the batch or
brand? PEG does oxidise over time.
Best,
Dave
--
Dr David C. Briggs
Senior Laboratory Research Scientist
Signalling and Structural Biology Lab
The Francis Crick Institute
London, UK
==
about.me/david_briggs
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