It's a very interesting crystallographic and likely biological problem.
These patterns--a series of peaks along the axes of the Chi=180 plot,
with the peaks corresponding to a great circle in polar (3D)
space--indicate 2 back-to-back rings. On each great circle (each line
of peaks on the
Applications are invited for a postdoctoral position in development and
application of methods for synchrotron-based serial and time resolved
crystallography of biomolecular systems. A joint project between Cornell
University and MiTeGen, LLC is developing tools for sample preparation,
A NIH-funded postdoctoral position is available immediately in the Department
of Biochemistry and Molecular Biology at University of Texas McGovern Medical
School in the laboratory of Dr. Lei Zheng to study the structure of a membrane
phosphatase protein and its novel regulatory mechanism. The
Hello Friends:
My lab has open positions for postdocs working on detergent-free membrane
protein structural biology.
The positions are supported by NIH grants and are available immediately. We
have been developing a detergent-free Native Cell Membrane Nanoparticles
system for membrane protein
Hi Peter,
We had a stash of them at Diamond. If you ask the i03 staff nicely, they should
be able to spare a couple.
Katherine
--
Katherine McAuley
Paul Scherrer Institut
WSLA/216
Forschungsstrasse 111
5232 Villigen PSI
From: CCP4 bulletin board on behalf of Peter Moody
Reply to: Peter
Hello,
has anyone in the UK a source for the little (~5 mm) clips that go on the
side of unipucks? Molecular Dimensions don't get back to me..I could order
from the USA, but it is a lot of bother and I'm too stingy to pay silly
shipping costs...I only need one, but more than happy to buy a few if
Dear Rakesh,
Congratulations that you have crystals in the first place!
I am sure you will hear this from others as well, but you did not tell us
how you tested your crystals for diffraction. It is not uncommon that
crystals diffract beautifully in their native environment of the
crystallisation
Our group has up to two openings for postdoctoral fellows to be funded on a
Cancer Research UK programme aimed at understanding lysosomal control of cell
proliferation and stress responses in cancer cells. The programme involves
cryo-electron microscopy, X-ray crystallography, H/D exchange mass
We have recently acquired a new diffraction setup and have retired our old
equipment.
We now have 2 Oxford cryostream 700 systems up for grabs. They come
complete with controller, dewar, pump, stand etc. Both work, but the
controllers have a few niggles that you need to know about - doesn't stop
Hi Rakesh,
The first question I have is: why do the crystals dissolve after some time? Did
you use a ligand for crystallization that might be turned-over by the protein?
What I would do first in your case is to try to find a stabilizing buffer.
Since you have obtained nice-looking crystals,
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