Re: [ccp4bb] am I doing this right?

2021-10-18 Thread Guillaume Gaullier
I am not qualified to comment on anything in the rest of this discussion, but regarding the excerpt quoted below: this way of recording an "image" seems very similar to the EER (electron event representation) of the Falcon 4 direct electron detector used in cryoEM. See https://doi.org/10.1107/S2

Re: [ccp4bb] am I doing this right?

2021-10-18 Thread James Holton
HDF5 is still "framing", but using better compression than the "byte offset" one implemented in Pilatus CBFs, which has a minimum of one byte per pixel. Very fast, but not designed for near-blank images. Assuming entropy-limited compression the ultimate data rate is the number of photons/s hit

Re: [ccp4bb] am I doing this right?

2021-10-18 Thread James Holton
Thank you very much for this Kay! So, to summarize, you are saying the answer to my question "what is the expectation and variance if I observe a 10x10 patch of pixels with zero counts?" is: Iobs = 0.01 sigIobs = 0.01 (defining sigIobs = sqrt(variance(Iobs))) And for the one-pixel case: I

[ccp4bb] Call for Registration to virtually attend Dr. Michael Rossmann Symposium on Oct 24-26, 2021

2021-10-18 Thread Xiao, Chuan
Dear CCP4 X-ray crystallographers, I am writing this email to invite you to attend Dr. Michael Rossmann Symposium that will be held on Oct 24-26, 2021. Dr. Michael Rossmann was a pioneer in protein crystallography and we hope you will be interested to come to the symposium in memory of him by

[ccp4bb] 4-year PhD position in Bergen, Norway

2021-10-18 Thread Petri Kursula
Hi, we have an opening for a fully funded 4-year PhD position in my group at hte Unviersity of Bergen, Norway. Please see the full ad below for details. Informal queries are welcome, but applications must be made through the link in the advert. https://www.jobbnorge.no/en/available-jobs/job/21

Re: [ccp4bb] am I doing this right?

2021-10-18 Thread Ian Tickle
All, this was my reply to one of James' emails which I just noticed was to me only, and I ought to have CC'd it to the BB, since it has relevance to others' contributions to the discussion. Cheers -- Ian On Mon, 18 Oct 2021 at 11:27, Ian Tickle wrote: > > James, no I don't think so, what does

[ccp4bb] Postdoc position Oxford Univ - Structural and Chemical Biology of E3 Ligases

2021-10-18 Thread Alex Bullock
A postdoc position is available in the group of Alex Bullock, University of Oxford, to study the structural and chemical biology of E3 Ligases as part of the EU-funded "EUbOpen" consortium, with funding until 30 April 2025. The project will support the next generation of PROTACs/molecular glues

Re: [ccp4bb] am I doing this right?

2021-10-18 Thread Kay Diederichs
Hi James, I'm a bit behind ... My answer about the basic question ("a patch of 100 pixels each with zero counts - what is the variance?") you ask is the following: 1) we all know the Poisson PDF (Probability Distribution Function) P(k|l) = l^k*e^(-l)/k! (where k stands for for an integer >=0

[ccp4bb] Bioinformatician position at PDBe in collaboration with CCP4

2021-10-18 Thread John Berrisford
Dear Colleagues, We have a bioinformatician position available in the PDBe team at the European Bioinformatics Institute (EBI) on the Wellcome Genome Campus near Cambridge. We are looking for a structural bioinformatician who is interested in developing methods for protein structure analysi