[ccp4bb] RapiData at SSRL 2024

Hi everyone, Applications are now open for the 2024 edition of RapiData at SSRL. RapiData is a practical course in macromolecular X-ray diffraction data collection, data processing and structure solution. The aim of the RapiData course is to educate and train young scientists in data collectio

Re: [ccp4bb] [OFF TOPIC] On Ni-NTA resin TEV cleavage

I have always performed TEV cleavage after eluting my protein from the Ni-NTA column (TEV cleavage performed during overnight dialysis in imidazole free buffer as imidazole inhibits TEV activity at high concentrations). I use TCEP as reducing agent and have never included EDTA, by this method I've

Re: [ccp4bb] [OFF TOPIC] On Ni-NTA resin TEV cleavage

Hi Rafael, In this case I recommend the use of Zn-charged Chelating Sepharose™ Fast Flow (Cytiva), as we have established it in the early days when first applying IMAC to the purification of native antibody fragments (see PMID: 1367302 and PMID: 8163179). While the Zn-IDA matrix has slightly

[ccp4bb] Obituaries for Michael James

For anyone who knew Michael James (who was my PhD supervisor), you might be interested in looking at two recent obituaries. One is in the current issue of ACA Reflexions (https://www.amercrystalassn.org/aca-reflexions), and the other was published in the October issue of Acta Cryst D (Structural

[ccp4bb] Postdoc positions available

POSTDOCTORAL POSITIONS at Brown University, USA The Deaconescu Lab at Brown University has two openings for postdoctoral researchers. The laboratory's interest is the interplay between DNA repair, transcription and other cellular processes ensuring microbial adaptation to stressful environm

[ccp4bb] New post-doc opportunity in RNA structure

Dear All, Please see the link below for details about a new post-doc (Research Fellow) position in RNA structure, based in the group of Prof. Teresa Carlomagno at the University of Birmingham. Research Fellow in RNA Structure

[ccp4bb] Senior Structural Biologist position at Exscientia

Dear all, We are seeking an experienced scientist specialising in Structural Biology to support the discovery of new therapeutic molecules the Structural Biology team at Exscientia. Exscientia is an AI-driven pharmatech company committed to discovering, designing and developing the best possible

Re: [ccp4bb] Fwd: radiation damage and image discard

Thanks, Clemens, yet more program/literature to study (and to come)! Jorge On 10/31/23 12:41, Clemens Vonrhein wrote: Dear Jorge, as Harry mentioned, autoPROC [1] will automatically exclude image ranges that are significantly worse than the rest (i.e. add mostly noise). This could be due to r

Re: [ccp4bb] [OFF TOPIC] On Ni-NTA resin TEV cleavage

Dear Rafael In addition to the excellent suggestions already offered by previous responders, I can attest that Ni-penta resin (sold among others by a company with an odd name Marvelgent) is resistant to EDTA and DTT, and it leaks very little Ni (almost none). Plus, it elutes with low inidazole, ow