> excess NaF and then used at say a 3:1 excess over ADP. Be sure to dissolve
>>> the chloride compounds in a fume hood!
>>>
>>> Also ADP-VO4, which can be prepared as described in Goodno CC. Myosin
>>> active-site trapping with vanadate ion. Meth Enzymol. 198
Hi
What are good ATP non hydrolyzable analog for crystallization?
best
Adriana
Dear Pierre
Daniel has raised a valid point either you use the same column for the
purification of mutant and wt. It happens in our case as well as we see the
activity of the inactive mutant but it is supposed to be caused by the
using the same column. Just compare the enzyme activity before perfor
Dear All
I am wondering if its possible to change the bottom filter of the sepharose
12 gel filteration column.
best
Adriana
Dear members
I assigned the secondary structure to my pdb file by the DSSP application
from this webserver
http://www.cmbi.ru.nl/dssp.html
The application works and gives output the pdb file.
The output file just have the reside list not the atom list. I am not able
to view the structure from thi
Hi
I am new here, I am looking for some way to modify one of my ligand. I have
some cl atoms in the ligand which I wanted to remove and add other
functional groups. If some one can tell me, how it can be done or which
tools either free or paid can be used to do that.
bet
Adiana
