Hello,
I have a 2.8 angstrom structure of a protein ligand complex. The R/R free
before adding the ligand was 0.21614/0.27760 and after adding the ligand,
the R/Rfree is 0.2030/0.2691.
.
However while refining the structure by Refmac 5, I see a decrease in R
factor but an increase in R free as
: 4.0.0
-- Forwarded message --
From: Amit Kumar amit7urmcc...@gmail.com
To: CCP4BB@JISCMAIL.AC.UK
Cc:
Date: Wed, 19 Mar 2014 21:46:13 +0530
Subject: Re: [ccp4bb] minimum acceptable sigma level for very small ligand
and more
Before putting in the ligand, there is a clear
Hello,
My protein is 26 kDa and the resolution of the data is 1.90 angs. My ligand
is 174 Daltons. and it was soaked into the crystal. Ligand is colored and
the crystal after soaking takes up intense color. However if we soak more
than optimum, the color deepens in intensity but the crystal
of the crystal.
Cheers,
Jared
--
Jared Sampson
Xiangpeng Kong Lab
NYU Langone Medical Center
550 First Avenue
New York, NY 10016
212-263-7898
http://kong.med.nyu.edu/
On Mar 19, 2014, at 10:39 AM, Amit Kumar amit7urmcc...@gmail.com wrote:
Hello,
My protein is 26 kDa
Hi all,
Apologies for non-ccp4 and very simple query.
How one will select and write into CNS input file
For example:
I would like to select following residues for omission to calculate SA-omit
map:
residue 10 to 15 of chain A and 12 to 17 of chain B
Thank you very much.
amit k
Hi all,
I am highly thankful to all of you for very useful replies.
The following are the list of the replies to the query.
Query:
Hi all,
Sorry for a non-ccp4 query.
I will highly appreciate your comments on the expression:
I am trying to express HIS tagged ~60 kDa cytosolic protein from
Hi all,
Sorry for a non-ccp4 query.
I will highly appreciate your comments on the expression:
I am trying to express HIS tagged ~60 kDa cytosolic protein from Bacillus in
E.coli pET expression system. I tried codon+ strain, time of expression
and various temperatures ( 18, 25, 30, 37C) for
