Hi,
I am just re-refining an old twinned dataset - which I will
redeposit (refinement is never finished).
The pdb seem to have automagically detwinned my data.
Where do I find my original twinned data (F and SIGF)? (or will I have
to redeposit my original un-detwinned structure factors).
Tha
Fcalc maps look fantastic. Are you sure they were not using an Fcalc for the
missing 35% of the data?
Ben
On 29 Feb 2024, at 21:33, Rafael Marques wrote:
Sorry for jumping into the post, but I would like the community’s opinion about
completeness, once this topic was raised here. W
Hi Kavya,
More than one crystal?
Epitaxial growth (protein crystal growing on salt crystal or vice versa)
Did you try IZIT?
(https://hamptonresearch.com/product-Izit-Crystal-Dye-33.html).
I have seen salt and protein crystals in the same drop before now - but not
growing together.
Ben
On 3
Hi Jorge,
I have had some similar cases.
The twin fraction can vary during data collection on the synchrotron
(different areas of the crystal can have different twin fractions).
Sometimes this is not a problem and twin refinement works fine - sometimes
it is a problem and it is hard to get a cle
The twin fraction can vary during data collection - if the beam is smaller than
the crystal
For example you could have crystals which are like two very short ends of
pencils - with flat ends together (in your case these might pencils could be in
P3)
In some orientations you could have the beam g
(and protonated)
forms of AMPPNP the same. When I tried to deposit a specific AMPPNP tautomer in
2013, they would not accept it. The PDB also seems to believe, as I understand
it, that the overall charge on AMPPNP is zero and that the phosphates do not
carry negative charge.
Ben Bax
Senior
pdb header.
Regards, Ben
Ben Bax
Senior Scientific Investigator
BioMolecular Sciences UK
RD Platform Technology & Science
GSK
Medicines Research Centre, Gunnels Wood Road, Stevenage, SG1 2NY, UK
Email benjamin.d@gsk.com<mailto:benjamin.d@gsk.com>
Mobile +44 (0) 7912
